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dc.contributor.authorFarré, A.-
dc.contributor.authorCuadrado, A.-
dc.contributor.authorLacasa-Benito, I.-
dc.contributor.authorCistué Sola, Luis-
dc.contributor.authorSchubert, I.-
dc.contributor.authorComadran, J.-
dc.contributor.authorJansen, J.-
dc.contributor.authorRomagosa, Ignacio-
dc.identifier.citationFarré A, Cuadrado A, Lacasa-Benito I, Cistué L, Schubert I, Comadran J, Jansen J, Romagosa I. Genetic characterization of a reciprocal translocation present in a widely grown barley variety. Molecular Breeding 30 (2): 1109-1119 (2012)es_ES
dc.description31 Pags., 2 Figs. The definitive version, with all tables and figures, is available at: http://link.springer.com/journal/11032es_ES
dc.description.abstractArtificially induced translocation stocks have been used to physically map the barley genome; however, natural translocations are extremely uncommon in cultivated genotypes. Albacete is a barley variety widely grown in recent decades in Spain and carrying a reciprocal translocation which obviously does not affect its agronomical fitness. This translocation has been characterized by a combination of cytological and molecular genetic approaches. Firstly, recombination frequencies between markers on chromosomes 1H and 3H were estimated to determine the boundaries of the reciprocal interchange. Secondly, 1H-3H wheat barley telosome addition lines were used to assign selected markers to chromosome arms. Thirdly, fluorescence in situ hybridization (FISH) with rDNA probes (5S and 18S-5.8S-26S) and microsatellite probes [(ACT)5, (AAG)5 and (CAG)5] was used to determine the locations of the translocation breakpoints more precisely. Fourthly, fine-mapping of the regions around the translocation breakpoints was used to increase the marker density for comparative genomics. The results obtained in this study indicate that the translocation is quite large with breakpoints located on the long arms of chromosomes 1H and 3H, between the pericentromeric (AAG)5 bands and above the (ACT)5 interstitial distal bands, resulting in the reciprocal translocation 1HS.1HL-3HL and 3HS.3HL-1HL. The gene content around the translocation breakpoints could be inferred from syntenic relationships observed among different species from the grass family Poaceae (rice, Sorghum and Brachypodium) and was estimated at approximately 1,100 and 710 gene models for 1H and 3H, respectively. Duplicated segments between chromosomes Os01 and Os05 in rice derived from ancestral duplications within the grass family overlap with the translocation breakpoints on chromosomes 1H and 3H in the barley variety Albacete.es_ES
dc.description.sponsorshipThis study has been supported by the Spanish Ministry of Science and Innovation (Project AGL2008-05541-C02 and the Agrogenomics CONSOLIDER Center).es_ES
dc.subjectReciprocal translocationes_ES
dc.subjectTranslocation breakpointes_ES
dc.subjectFluorescence in situ hybridizationes_ES
dc.subjectComparative genomicses_ES
dc.titleGenetic characterization of a reciprocal translocation present in a widely grown barley varietyes_ES
dc.description.peerreviewedPeer reviewedes_ES
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