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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/55386
Title: Inhibition of ∆9-desaturase activity with sterculic acid: Effect on the endogenous synthesis of cis-9 18:1 and cis-9 trans-11 18:2 in dairy sheep
Authors: Bichi, Elena; Toral, Pablo G.; Hervás, Gonzalo; Frutos, Pilar; Gómez-Cortés, Pilar; Juárez, Manuela; Fuente, Miguel Ángel de la
Keywords: Desaturase system
Cyclopropene fatty acid
Oleic acid
Rumenic acid
Issue Date: Sep-2012
Publisher: Elsevier
Citation: Journal of Dairy Science 95(9): 5242-5252 (2012)
Abstract: This study was conducted in lactating ewes to examine the involvement of Δ9-desaturase in mammary lipogenesis, especially in the endogenous synthesis of cis-9, trans-11 18:2 and cis-9 18:1, because no information on this matter was available for dairy sheep. With this aim, 6 Assaf ewes were monitored in a 15-d experiment, which included a 5-d pretreatment period, a 5-d treatment period, and a 5-d posttreatment period. During the treatment period, ewes received 0.5 g/d of sterculic acid (a cyclopropene fatty acid that inhibits Δ9-desaturase), delivered intravenously in 4 equal doses at 6-h intervals. Animals were fed pasture to supply mainly α-linolenic acid and minimize the amount of milk cis-9, trans-11 18:2 of ruminal origin. Sterculic acid administration was calculated to inhibit Δ9-desaturase by 70% based on the milk content of cis-9 14:1. This inhibition resulted in decreases in the milk content of the enzyme products (e.g., cis-9 10:1, cis-9 14:1, cis-9 16:1, cis-9 18:1, and cis-9, trans-11 18:2) and increases in its substrates (e.g., 14:0, 18:0, and trans-11 18:1), as well as in reductions in the desaturase indexes. Some other milk fatty acids, further to previously reported products or substrates of Δ9-desaturase (e.g., cis-15 18:1 and cis-9, cis-15 18:2, or trans-11, trans-15 18:2, and cis-9, trans-11, trans-15 18:3), were also affected by sterculic acid administration. Endogenous synthesis was the major source of cis-9 18:1 and cis-9, trans-11 18:2, accounting for 63 and 74% of its content in milk fat, respectively. To our knowledge, the present study provides the first estimates of endogenous synthesis of these 2 bioactive fatty acids in ovine milk fat.
Description: 11 páginas, 2 figuras, 4 tablas.
Publisher version (URL): http://dx.doi.org/10.3168/jds.2012-5349
URI: http://hdl.handle.net/10261/55386
ISSN: 0022-0302
DOI: 10.3168/jds.2012-5349
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