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Title

Comparative methods for the evaluation of protein adsorption

AuthorsServoli, Eva; Maniglio, Devid; Aguilar, María Rosa ; Motta, Antonella; Vázquez Lasa, Blanca; San Román, Julio ; Migliaresi, Claudio
Keywordsadsorption
atomic force microscopy (AFM)
biological applications of polymers
proteins
surfaces
Issue Date2009
PublisherWiley-VCH
CitationMacromolecular bioscience 9 (7) : 661-679 (2009)
AbstractAFM, Immunogold labeling and SPR have been successfully applied to the analysis of protein adsorption on fibroin substrates, chosen as a paradigmatic biomaterial. The process was evaluated both under static and dynamic conditions, also considering the effect of protein competition. AFM via antibody-modified tips provided maps of protein adsorption and information on protein-protein interactions. Double labeling Immunogold imaged protein distribution upon competitive adsorption. SPR described the dynamics of the process, offering also quantitative data both from individual and two-component protein solutions. The integration of these analytical techniques and the critical evaluation of the results can provide a detailed description of protein adsorption.
Publisher version (URL)http://dx.doi.org/10.1002/mabi.200800301
URIhttp://hdl.handle.net/10261/55283
DOI10.1002/mabi.200800301
ISSN1616-5187
E-ISSN1616-5195
Appears in Collections:(ICTP) Artículos
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