English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/55194
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Effect of the ferredoxin electron donor on sunflower (Helianthus annuus) desaturases

AuthorsVenegas-Calerón, Mónica ; Youssar, Loubna ; Salas, Joaquín J. ; Garcés Mancheño, Rafael ; Martínez-Force, Enrique
KeywordsFerredoxin
Photosynthetic
Heterotrophic
Stearoyl-ACP desaturase
Membrane bound desaturases
FAD7
Helianthus annuus
Issue DateAug-2009
PublisherElsevier
CitationPlant Physiology and Biochemistry 47(8): 657-662 (2009)
AbstractFerredoxins are proteins that participate in photosynthesis and in other processes that require reducing equivalents, such as the reduction of nitrogen or fatty acid desaturation. Two classes of ferredoxins have been described in plants: light-regulated photosynthetic ferredoxins and heterotrophic ferredoxins whose activity is not influenced by light. Genes encoding the two forms of ferredoxin have been cloned and characterized in developing sunflower cotyledons. Here, these genes were overexpressed in Escherichia coli and they were purified by ion exchange and size exclusion chromatography to study their capacity to supply electrons to two different sunflower desaturases: soluble stearoyl-ACP desaturase from sunflower cotyledons, and membrane bound desaturase FAD7 expressed in yeast. In both cases photosynthetic ferredoxin was the form that promoted the strongest desaturase activity.
Publisher version (URL)http://dx.doi.org/10.1016/j.plaphy.2009.03.005
URIhttp://hdl.handle.net/10261/55194
DOI10.1016/j.plaphy.2009.03.005
ISSN0981-9428
Appears in Collections:(IG) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.