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Evidence of high individual diversity on myticin C in mussel (Mytilus galloprovincialis)

AuthorsCosta, M. M. ; Dios, S. ; Alonso-Gutiérrez, Jorge ; Romero Jódar, Alejandro ; Novoa, Beatriz ; Figueras Huerta, Antonio
Antimicrobial peptides
Mytilus galloprovincialis
Issue Date2009
CitationDevelopmental and Comparative Immunology 33(2): 162-170 (2009)
AbstractSeveral antimicrobial peptides (AMP) have been described in Mytilus galloprovincialis. However, only in myticin C a high variability on the nucleotide sequence was detected. To determine the individual variability of this AMP, the myticin C present in more than 100 mussels was analyzed by denaturing gradient gel electrophoresis (DGGE). This technique helped us to describe a very highmyticin C diversity as compared with a non-immune related gene such as the b-actin. Moreover, each mussel showed a specific and exclusive myticin C band pattern. Our results showed that the individual sequences of myticin C are unique for each mussel, independently of their geographic origin, age, sex, gonad maturation stage or aggregate where they group together on the wild. Only the animals belonging to the same family shared myticin C sequences. The comparative analysis of genomic DNA and cDNA sequences from the same individual showed that all detected variants shared a very high homology with the more frequent genomic isoforms, suggesting that all the variations were generated from the more common sequences, through a mechanism not yet determined. The fact that myticin C possesses characteristics of an immune gene, its potential antimicrobial effects, molecular diversity, as well as its early and ubiquitous expression, led us to suggest that myticin C might play an important role in innate immune defense in mussels.
Description9 páginas, 6 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.dci.2008.08.005
Appears in Collections:(IIM) Artículos
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