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Título: | Evaluation of a novel 16S rRNA/tRNAVal mitochondrial marker for the identification and phylogenetic analysis of shrimp species belonging to the superfamily Penaeoidea |
Autor: | Calo-Mata, Pilar CSIC; Pascoal, Ananías; Fernández-No, Inmaculada C.; Böhme, K.; Gallardo, José Manuel CSIC; Barros-Velázquez, Jorge CSIC ORCID | Palabras clave: | Mitochondrial DNA Penaeid shrimp Decapoda 16S rRNA Cytochrome c oxidase I (COI) Species identification Food authenticity PCR–RFLP Phylogenetic analysis |
Fecha de publicación: | 2009 | Editor: | Elsevier | Citación: | Analytical Biochemistry 391(2): 127-134 (2009) | Resumen: | In this study, we infer the phylogenetic relationships within commercial shrimp using sequence data from a novel mitochondrial marker consisting of an approximately 530-bp region of the 16S ribosomal RNA (rRNA)/transfer RNA (tRNA)Val genes compared with two other mitochondrial genes: 16S rRNA and cytochrome c oxidase I (COI). All three mitochondrial markers were considerably AT rich, exhibiting values up to 78.2% for the species Penaeus monodon in the 16S rRNA/tRNAVal genes, notably higher than the average among other Malacostracan mitochondrial genomes. Unlike the 16S rRNA and COI genes, the 16S rRNA/tRNAVal marker evidenced that Parapenaeus is more closely related to Metapenaeus than to Solenocera, a result that seems to be more in agreement with the taxonomic status of these genera. To our knowledge, our study using the 16S rRNA/tRNAVal gene as a marker for phylogenetic analysis offers the first genetic evidence to confirm that Pleoticus muelleri and Solenocera agassizi constitute a separate group and that they are more related to each other than to genera belonging to the family Penaeidae. The 16S rRNA/tRNAVal region was also found to contain more variable sites (56%) than the other two regions studied (33.4% for the 16S rRNA region and 42.7% for the COI region). The presence of more variable sites in the 16S rRNA/tRNAVal marker allowed the interspecific differentiation of all 19 species examined. This is especially useful at the commercial level for the identification of a large number of shrimp species, particularly when the lack of morphological characteristics prevents their differentiation. | Descripción: | 8 páginas, 4 figuras, 5 tablas | Versión del editor: | http://dx.doi.org/10.1016/j.ab.2009.05.020 | URI: | http://hdl.handle.net/10261/54950 | DOI: | 10.1016/j.ab.2009.05.020 | ISSN: | 0003-2697 |
Aparece en las colecciones: | (IIM) Artículos |
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