Recursos Naturales >
Instituto de Investigaciones Marinas (IIM) >
(IIM) Artículos >
Closed Access item Dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of Senegalese sole (Solea senegalensis Kaup, 1858)
Conceiçao, Luís E. C.
|Keywords:||Bacterial challenge, Immune-related genes, Nitric oxide, Respiratory burst activity, Stress response|
|Citation:||Fish and Shellfish Immunology 31(6): 838-847 (2011)|
|Abstract:||Stress is known to impair immune function and disease resistance in fish. In the present study, repeated
handling was employed as a chronic stressor in order to verify whether its attributed immunosuppressive
effects could be minimized by dietary arginine supplementation. Therefore, Senegalese sole (Solea
senegalensis) were air exposed daily for 3 min during 14 days (handling) or left undisturbed (control).
In addition, both control and handled specimens were fed 3 diets with graded levels of arginine (Arg 4.4,
Arg 5.7 and Arg 6.9 g 16 g 1 N). Following the 14 days stress challenge and feeding on those diets, fish were
infected with Photobacterium damselae subsp. piscicida (strain PC566.1; LD50 5 103 cfu mL 1) and fed the
same experimental diets. Respiratory burst activity and nitric oxide production of headekidney leucocytes
increased parallel to dietary arginine supplementation. HIF-1, HAMP-1, MIP1-alpha and gLYS expression
values and some humoral parameters augmented in control specimens fed the Arg 5.7 and Arg 6.9 diets.
Interestingly, repeated acute stress increased both disease resistance and some innate immune mechanisms
in handled fish. The role of dietary arginine and repeated handling on Senegalese sole innate
immunity and disease resistance are discussed.|
|Description:||10 páginas, 4 figuras, 4 tablas|
|Publisher version (URL):||http://dx.doi.org/10.1016/j.fsi.2011.07.024|
|Appears in Collections:||(IIM) Artículos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.