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Título: | A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans |
Autor: | Muñoz-Pajares, A. J.; Martínez-Rodríguez, L.; Teruel, María; Cabrero, J.; Camacho, Juan Pedro M.; Perfectti, F. | Fecha de publicación: | 2011 | Editor: | Genetics Society of America | Citación: | Genetics 187: 853- 863 (2011) | Resumen: | B chromosomes are dispensable chromosomes found in >2000 eukaryotic species, usually behaving as genomic parasites. Most B chromosomes seem to be made up of the same kind of DNA sequences present in the A chromosomes. This sequence similarity makes it difficult to obtain specific molecular probes that may permit B-presence diagnosis without cytogenetic analysis. We have developed a sequence-characterized amplified region (SCAR) marker for B chromosomes in the grasshopper Eyprepocnemis plorans, which specifically amplifies a 1510-bp DNA fragment exclusively in B-carrying individuals. Fluorescent in situ hybridization and fiber FISH analyses showed that this marker is a tandemly repeated DNA sequence closely intermingled with 45S rDNA. PCR reactions showed the presence of SCAR-like sequences in the A chromosomes, but in two separate fragments, supporting the intraspecific origin of B chromosomes in this species. SCAR marker DNA sequence showed to be identical in B chromosome variants from several localities from Spain and Morocco, and it was very similar to those found in B chromosome variants from Greece and Armenia. This strongly suggests that this sequence was already present in the ancestral B chromosome of this species. In addition, the scarce sequence variation observed among several B variants from very distant populations suggests either a functional constraint or, more likely, a recent and unique origin for B chromosomes in this species. Copyright © 2011 by the Genetics Society of America. | URI: | http://hdl.handle.net/10261/54259 | DOI: | 10.1534/genetics.110.122713 | Identificadores: | doi: 10.1534/genetics.110.122713 issn: 0016-6731 |
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