Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications

Abstract

Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides carrying peptide sequences at the 3¢-end on a single support. The resulting oligonucleotide–peptide conjugates may be used as exogenous effectors for the specific control of gene expression. In the second part of this chapter, detailed postsynthetic conjugation protocols to introduce peptide sequences into oligonucleotide sequences are also presented.