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dc.contributor.authorAlcolea, Pedro J.-
dc.contributor.authorAlonso, Ana-
dc.contributor.authorSánchez-Gorostiaga, Alicia-
dc.contributor.authorMoreno-Paz, Mercedes-
dc.contributor.authorGómez, Manuel José-
dc.contributor.authorRamos, Irene-
dc.contributor.authorParro, Víctor-
dc.contributor.authorLarraga, Vicente-
dc.identifier.citationGenomics 93(6):551-564(2009)es_ES
dc.description14 páginas, 5 figuras, 2 tablas -- PAGS nros. 551-564es_ES
dc.description.abstractMetacyclic promastigotes are transmitted during bloodmeals after development inside the gut of the sandfly vector. The isolation from axenic cultures of procyclic and metacyclic promastigotes by peanut lectin agglutination followed by differential centrifugation is controversial in Leishmania infantum. The purpose of this study has been to isolate both fractions simultaneously from the same population in stationary phase of axenic culture and compare their expression profiles by whole-genome shotgun DNA microarrays. The 317 genes found with meaningful values of stage-specific regulation demonstrate that negative selection of metacyclic promastigotes by PNA agglutination is feasible in L. infantum and both fractions can be isolated. This subpopulation up-regulates a cysteine peptidase A and several genes involved in lipophosphoglycan, proteophosphoglycan and glycoprotein biosynthesis, all related with infectivity. In fact, we have confirmed the increased infection rate of PNA(-) promastigotes by U937 human cell line infection experiments. These data support that metacyclic promastigotes are related with infectivity and the lack of agglutination with PNA is a phenotypic marker for this subpopulationes_ES
dc.description.sponsorshipThis work has been supported by Ministerio de Educación y Ciencia (MEC) project AGL2003–06152-C02–01 and Centro de Astrobiología internal budget. PJA thanks MEC for I3P-BPD2003–1 grant and AA and IR for a contract. MMP and MG are personnel from INTA and VP has a “Ramón y Cajal” contract from MEC. ASG thanks Ministerio de Ciencia y Tecnología for BMC 2003–00088 project and Comunidad Autónoma de Madrid for GR/SAL/06512004 projectes_ES
dc.subjectLeishmania infantumes_ES
dc.subjectShotgun DNA microarrayses_ES
dc.subjectPeanut lectin agglutinationes_ES
dc.subjectMetacyclic promastigoteses_ES
dc.titleGenome-wide analysis reveals increase levels of transcripts related with infectivity in peanut lectin non-agglutinated promastigotes of Leishmania infantumes_ES
dc.description.peerreviewedPeer reviewedes_ES
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