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Título

Assessing carbohydrate-carbohydrate interactions by NMR spectroscopy: the trisaccharide epitope from the marine sponge Microciona prolifera

AutorSantos, José Ignacio ; Carvalho de Souza, Adriana; Cañada, F. Javier ; Martín-Santamaría, Sonsoles; Kamerling, Johannis P.; Jiménez-Barbero, Jesús
Palabras clavecarbohydrate–carbohydrate interactions
gold glyconanoparticles
Microciona prolifera
molecular dynamics
NMR spectroscopy
Fecha de publicación13-feb-2009
EditorWiley-VCH
CitaciónChemBioChem 10(3):511-519(2009)
ResumenWeak recognition processes: Weak calcium-mediated carbohydrate–carbohydrate interactions have been detected by DOSY and TRNOESY NMR methods by employing a gold glyconanoparticle as a multivalent system. In addition, 3D models of trisaccharide-CaII-trisaccharide complexes based on results from molecular dynamics simulations are proposed. Diffusion-ordered NMR spectroscopy (DOSY-NMR) and TR-NOESY-NMR experiments are used to detect ligand binding to macromolecular receptors. These techniques have been applied to detect weak carbohydrate–carbohydrate self-recognition in solution, making use of sugar-decorated gold nanoparticles as the “macromolecule” and the same carbohydrate as the ligand. Changes in the diffusion coefficient of the free carbohydrate in the presence of the glyconanoparticle (only with CaII ions in the sample solution), as well as changes in the sign of the sugar NOE peaks—positive for the free sugar (in the presence or absence of CaII) and negative for the sugar only in the simultaneous presence of the glyconanoparticle and CaII ions—have been taken as proof of weak CaII-mediated carbohydrate–carbohydrate interactions in solution. Although different methods such as SPR, TEM, and AFM have been used in the past to detect carbohydrate–carbohydrate interactions with the aid of gold nanoparticles and gold selfassembled monolayers, they are restricted to high-affinity ranges. The methods used in this study allow expansion of the number of techniques to tackle this relevant biological problem, also for approaching ligand–receptor interactions below the high-affinity range. Additionally, 3D models of trisaccharide-CaII-trisaccharide complexes based on results from molecular dynamics simulations are proposed
Descripción9 páginas, 7 figuras, 2 tablas, 1 esquema -- PAGS nros. 511-519
Versión del editorhttp://dx.doi.org./10.1002/cbic.200800548
URIhttp://hdl.handle.net/10261/53957
DOI10.1002/cbic.200800548
ISSN1439-4227
E-ISSN1439-7633)
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