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Title

Antiproliferative and apoptosis-inducing effects of maslinic and oleanolic acids, two pentacyclic triterpenes from olives, on HT-29 colon cancer cells

AuthorsJuan, M. Emilia; Planas, Joana M.; Ruiz-Gutiérrez, Valentina ; Daniel, Hannelore; Wenzel, Uwe
KeywordsMaslinic acid
Oleanolic acid
HT-29 cells
Issue DateJul-2008
PublisherCABI Publishing
CitationBritish Journal of Nutrition 100(1): 36-43 (2008)
AbstractWe have previously reported the anticarcinogenic effects of an olive fruit extract composed of pentacyclic triterpenes, the main components of which are maslinic acid (73.25%) and oleanolic acid (25.75%). Here we examined the effects of the individual components on proliferation, necrosis and apoptosis rates by fluorescence-based techniques in human HT-29 colon cancer cells. Oleanolic acid showed moderate antiproliferative activity, with an EC50 of 160.6 (SE 10.6) μmol/l, and moderate cytotoxicity at high concentrations (≥250 μmol/l). On the other hand, maslinic acid inhibited cell growth with an EC50 of 101.2 (SE 7.8) μmol/l, without necrotic effects. Oleanolic acid, which lacks a hydroxyl group at the carbon 2 position, failed to activate caspase-3 as a prime apoptosis protease. In contrast, maslinic acid increased caspase-3-like activity at 10, 25 and 50 μmol/l by 3-, 3.5- and 5-fold over control cells, respectively. The detection of ROS in the mitochondria, which serve as pro-apoptotic signal, evidenced the different bioactivity of the two triterpenes. Confocal microscopy analysis revealed that maslinic acid generated superoxide anions while oleanolic acid-treated cells did not differ from the control. Completion of apoptosis by maslinic acid was confirmed microscopically by the increase in plasma membrane permeability, and detection of DNA fragmentation. In conclusion, the anticancer activity observed for olive fruit extracts seems to originate from maslinic acid but not from oleanolic acid. Maslinic acid therefore is a promising new compound for the chemoprevention of colon cancers. © The Authors 2008.
URIhttp://hdl.handle.net/10261/53542
DOI10.1017/S0007114508882979
Identifiersdoi: 10.1017/S0007114508882979
issn: 0007-1145
e-issn: 1475-2662
Appears in Collections:(IG) Artículos
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