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Glycan tagging to produce bioactive ligands for a surface plasmon resonance study via immobilization on different surfaces

AuthorsMuñoz, F. Javier; Pérez, José; Rumbero, Ángel; Santos, José Ignacio ; Cañada, F. Javier ; André, Sabine; Gabius, Hans-Joachim; Jiménez-Barbero, Jesús ; Sinisterra, José V.; Hernáiz, María J.
Issue DateApr-2009
PublisherAmerican Chemical Society
CitationBioconjugate Chemistry 20(4):673-682(2009)
AbstractSuitable glycan derivatives will find immediate application in the study of their interactions. Here, we present an efficient synthetic strategy to introduce a fluorescent tag functionalized with an amino group into a model disaccharide structure (lactose). This strategy led to the maintenance of bioactivity, checked by the study of the interaction of this bioconjugate with a plant lectin (mistletoe lectin 1) by NMR spectroscopy, computational docking, and surface plasmon resonance (SPR). To demonstrate the versatility of this approach, we immobilized the new glycan derivatives on different surfaces, and a comparative analysis is presented and can be successfully used for biomimetic carbohydrate−protein interaction studies on the SPR biochip
Description10 páginas, 9 figuras, 2 tablas, 2 esquemas -- PAGS nros. 673-682
Publisher version (URL)http://dx.doi.org/10.1021/bc800350q
Appears in Collections:(CIB) Artículos
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