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Título : Glycan tagging to produce bioactive ligands for a surface plasmon resonance study via immobilization on different surfaces
Autor : Muñoz, F. Javier, Pérez, José, Rumbero, Ángel, Santos, José Ignacio, Cañada Vicinay, Francisco Javier, André, Sabine, Gabius, Hans-Joachim, Jiménez-Barbero, Jesús, Sinisterra, José V., Hernáiz, María J.
Fecha de publicación : Apr-2009
Editor: American Chemical Society
Resumen: Suitable glycan derivatives will find immediate application in the study of their interactions. Here, we present an efficient synthetic strategy to introduce a fluorescent tag functionalized with an amino group into a model disaccharide structure (lactose). This strategy led to the maintenance of bioactivity, checked by the study of the interaction of this bioconjugate with a plant lectin (mistletoe lectin 1) by NMR spectroscopy, computational docking, and surface plasmon resonance (SPR). To demonstrate the versatility of this approach, we immobilized the new glycan derivatives on different surfaces, and a comparative analysis is presented and can be successfully used for biomimetic carbohydrate−protein interaction studies on the SPR biochip
Descripción : 10 páginas, 9 figuras, 2 tablas, 2 esquemas -- PAGS nros. 673-682
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ISSN: 1043-1802
DOI: 10.1021/bc800350q
Citación : Bioconjugate Chemistry 20(4):673-682(2009)
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