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Title

A combinatorial approach for the design of complementarity determining regions (CDR)-derived peptidomimetics with in vitro anti-tumoral activity

AuthorsTimmerman, Peter; Barderas, Rodrigo ; Desmet, Johan; Altschuh, Danièle; Shochat, Susana Geifman; Hollestelle, Martine J.; Höppener, Jo W.M.; Monasterio, Alberto; Casal, J. Ignacio ; Meloen, Rob H.
Issue Date4-Dec-2009
CitationJournal of Biological Chemistry 284(49):34126-34134(2009)
AbstractThe great success of therapeutic monoclonal antibodies has fueled research toward mimicry of their binding sites and the development of new strategies for peptide-based mimetics production. Here, we describe a new combinatorial approach for the production of peptidomimetics using the complementarity-determining regions (CDRs) from gastrin17 (pyroEGPWLEEEEEAYGWMDF-NH(2)) antibodies as starting material for cyclic peptide synthesis in a microarray format. Gastrin17 is a trophic factor in gastrointestinal tumors, including pancreatic cancer, which makes it an interesting target for development of therapeutic antibodies. Screening of microarrays containing bicyclic peptidomimetics identified a high number of gastrin binders. A strong correlation was observed between gastrin binding and overall charge of the peptidomimetic. Most of the best gastrin binders proceeded from CDRs containing charged residues. In contrast, CDRs from high affinity antibodies containing mostly neutral residues failed to yield good binders. Our experiments revealed essential differences in the mode of antigen binding between CDR-derived peptidomimetics (K(d) values in micromolar range) and the parental monoclonal antibodies (K(d) values in nanomolar range). However, chemically derived peptidomimetics from gastrin binders were very effective in gastrin neutralization studies using cell-based assays, yielding a neutralizing activity in pancreatic tumoral cell lines comparable with that of gastrin-specific monoclonal antibodies. These data support the use of combinatorial CDR-peptide microarrays as a tool for the development of a new generation of chemically synthesized cyclic peptidomimetics with functional activity
Description9 páginas, 6 figuras, 1 tabla -- PAGS nros. 34126-34134
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M109.041459
URIhttp://hdl.handle.net/10261/53457
DOI10.1074/jbc.M109.041459
ISSN0021-9258
E-ISSN1083-351X
Appears in Collections:(CIB) Artículos
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