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Título: | The organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosis |
Autor: | Pantazopoulou, Areti CSIC ORCID; Peñalva, Miguel Ángel CSIC ORCID | Fecha de publicación: | 15-oct-2009 | Editor: | American Society for Cell Biology | Citación: | Molecular Biology of the Cell 20(20):4335-4337(2009) | Resumen: | Aspergillus nidulans hyphae grow exclusively by apical extension. Golgi equivalents (GEs) labeled with mRFP-tagged PHOSBP domain form a markedly polarized, dynamic network of ring-shaped and fenestrated cisternae that remains intact during “closed” mitosis. mRFP-PHOSBP GEs advance associated with the growing apex where secretion predominates but do not undergo long-distance movement toward the tip that could account for their polarization. mRFP-PHOSBP GEs overlap with the trans-Golgi resident Sec7 but do not colocalize with also polarized accretions of the early Golgi marker GrhAGrh1-GFP, indicating that early and late Golgi membranes segregate spatially. AnSec23-GFP ER exit sites (ERES) are numerous, relatively static foci localizing across the entire cell. However, their density is greatest near the tip, correlating with predominance of early and trans-Golgi elements in this region. Whereas GrhA-GFP structures and ERES reach the apical dome, mRFP-PHOSBP GEs are excluded from this region, which contains the endosome dynein loading zone. After latrunculin-mediated F-actin disruption, mRFP-PHOSBP GEs fragment and, like AnSec23-GFP ERES, depolarize. Brefeldin A transiently collapses late and early GEs into distinct aggregates containing Sec7/mRFP-PHOSBP and GrhA-GFP, respectively, temporarily arresting apical extension. Rapid growth reinitiates after washout, correlating with reacquisition of the normal Golgi organization that, we conclude, is required for apical extension | Descripción: | 3 páginas 10 figuras -- PAGS nros. 4335-4337 | Versión del editor: | http://dx.doi.org/10.1091/mbc.E09-03-0254 | URI: | http://hdl.handle.net/10261/53449 | DOI: | 10.1091/mbc.E09-03-0254 | ISSN: | 10.1091/mbc.E09-03-0254 | E-ISSN: | 1939-4586) |
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Mol. Biol Cell, 20 (4335-4337).pdf | 2,63 MB | Adobe PDF | Visualizar/Abrir |
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