English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/53441
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit

AuthorsHernández, M. Luisa ; Padilla, María Nieves ; Sicardo, M. Dolores ; Mancha Perello, Manuel ; Martínez-Rivas, José Manuel
KeywordsOlea europaes
Oleae
Olive
Gene expression
Olea desaturase
Oleic acid
Linoleic acid
Palmotolinoleic acid
FAD2
FAD6
Issue DateFeb-2011
PublisherElsevier
CitationPhytochemistry 72(2-3): 178-187 (2011)
AbstractThe regulation of microsomal and plastidial oleate desaturases by low and high temperature, darkness, and wounding was investigated. To this end, their gene expression levels and the fatty acid composition was determined in the mesocarp tissue of olive fruit from the Picual and Arbequina varieties subjected to the corresponding stress treatments. Firstly, a plastidial oleate desaturase from olive was cloned and its functional identity was confirmed by overexpression in Escherichia coli. The results showed that temperature and light regulate olive oleate desaturase genes at transcriptional level. However, no correlation between their expression levels and the linoleic acid content in microsomal and plastidial lipids was found. In addition, the involvement of microsomal but not plastidial oleate desaturases in the wounding response of olive fruit mesocarp is demonstrated. The fatty acid analysis revealed the appearance of palmitolinoleic acid only in microsomal lipids, reaching a maximum 3 h after wounding. © 2010 Elsevier Ltd. All rights reserved.
URIhttp://hdl.handle.net/10261/53441
DOI10.1016/j.phytochem.2010.11.026
Identifiersdoi: 10.1016/j.phytochem.2010.11.026
issn: 0031-9422
Appears in Collections:(IG) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.