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dc.contributor.authorGonzález, Inmaculada-
dc.contributor.authorMartínez-Priego, Llucia-
dc.contributor.authorRakitina, Daria V.-
dc.contributor.authorLewsey, Mathew-
dc.contributor.authorAtencio, Félix A.-
dc.contributor.authorLlave, César-
dc.contributor.authorKalinina, Natalia O.-
dc.contributor.authorCarr, John P.-
dc.contributor.authorPalukaitis, Peter-
dc.contributor.authorCanto, Tomás-
dc.identifier.citationMolecular Plant-Microbe Interactions 23(3):294-303(2010)es_ES
dc.description10 páginas, 8 figuras -- PAGS nros. 294-303es_ES
dc.description.abstractThe RNA silencing suppressor activity of the 2b protein of Cucumber mosaic virus (CMV) has been variously attributed to its nuclear targeting, its interaction with and inhibition of Argonaute 1 (AGO1), or its ability to bind small RNAs in vitro. In addition, the 2b ortholog of Tomato aspermy virus forms aggregates and binds RNAs in vitro. We have further studied the relationships between CMV 2b protein silencing suppressor activity and its subcellular distribution, protein-protein interactions in vivo, and interactions with small interfering RNAs in vitro. To do this, we tagged the protein with fluorescent markers and showed that it retained suppressor activity. We showed that the 2b protein is present in the nucleolus and that it self-interacts and interacts with AGO1 and AGO4 in vivo. Using a battery of mutants, we showed that the putative nuclear localization signals and phosphorylation motif of the 2b protein are not required for self-interaction or for interaction with AGO proteins. The occurrence of neither of these interactions or of nucleolar targeting was sufficient to provide local silencing-suppression activity. In contrast, the ability of the 2b protein to bind small RNAs appears to be indispensable for silencing suppressor functiones_ES
dc.description.sponsorshipKim for providing the binary construct expressing fibrillarin-mRFP. Work at T. C Canto’s lab was funded by grants 200740I003 from the CSIC, grant L2008-03482 from the Spanish Ministry of Innovation and Science, and bilateral grant P2008RU03 from the Spanish Ministry of Innovation and Science and the Russian Foundation for Basic Research. I. González s recipient of a Ph.D. grant from the Spanish Ministry of Innovation and Science. L. Martínez, C. Llave, and F. Atencio were funded by the Spanish Ministry of Innovation and Science. P. Palukaitis was funded by the Scottish Executive Research Evaluation and Rural Affairs Directorate. M. G. Lewsey and J. P. Carr were funded by Biotechnology and Biological Sciences Research Council grant BB/D008204/1es_ES
dc.publisherAmerican Phytopathological Societyes_ES
dc.titleCucumber Mosaic Virus 2b Protein Subcellular Targets and Interactions: Their Significance to RNA Silencing Suppressor Activityes_ES
dc.description.peerreviewedPeer reviewedes_ES
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