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Title

Acyl-ACP thioesterases from macadamia (Macadamia tetraphylla) nuts: Cloning, characterization and their impact on oil composition

AuthorsMoreno-Pérez, Antonio J.; Sánchez-García, Alicia ; Salas, Joaquín J. ; Garcés Mancheño, Rafael ; Martínez-Force, Enrique
KeywordsAcyl-ACP thioeserase
Aclepic acid
Beaumont
Cate
Macadamia tetraphylla
Palmitoleic acid
Issue DateJan-2011
PublisherElsevier
CitationPlant Physiology and Biochemistry 49(1): 82-87 (2011)
AbstractThe mechanisms by which macadamia nuts accumulate the unusual palmitoleic and asclepic acyl moieties, which constitute up to 20% of the fatty acids in some varieties, are still unknown. Acyl-acyl carrier protein (ACP) thioesterases (EC 3.1.2.14) are intraplastidial enzymes that terminate the synthesis of fatty acids in plants and that facilitate the export of the acyl moieties to the endoplasmic reticulum where they can be used in the production of glycerolipids. Here, we have investigated the possible role of acyl-ACP thioesterase activity in the composition of macadamia kernel oil. Accordingly, two acyl-ACP thioesterases were cloned from developing macadamia kernels, one of the FatA type and the other of the FatB type. These enzymes were heterologously expressed in Escherichia coli, and the recombinant thioesterases were purified, characterized kinetically and assayed with a variety of substrates, demonstrating the high specificity of macadamia FatA towards 16:1-ACP. Acyl-ACP thioesterase activity was also characterized in crude extracts from two different varieties of macadamia, Cate and Beaumont, which accumulate different amounts of n-7 fatty acids. The impact of acyl-ACP thioesterase activities on the oil composition of these kernels is discussed in the light of these results. © 2010 Elsevier Masson SAS.
URIhttp://hdl.handle.net/10261/53324
DOI10.1016/j.plaphy.2010.10.002
Identifiersdoi: 10.1016/j.plaphy.2010.10.002
issn: 0981-9428
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