Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/53298
Share/Impact:
Título : Transcriptional and proteomic profiling of flatfish (Solea senegalensis) spermatogenesis
Autor : Forné, Ignasi, Castellana, Bárbara, Marín-Juez, Rubén, Cerdà, Joan, Abián, Joaquín, Planas, Josep V.
Palabras clave : Animal proteomics
Flatfish
Gene expression
Spermatogenesis
Testis
Fecha de publicación : Jun-2011
Editor: Wiley-VCH
Resumen: The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. The efforts to reproduce this species in captivity have been hampered by the fact that farmed males (F1) often show lower sperm production and fertilization capacity than wild-type males (F0). Our knowledge on spermatogenesis is however limited to a few studies. In a previous work, we identified by 2-D DIGE several potential protein markers in testis for the poor reproductive performance of F1 males. Therefore, the objectives of the present study were, first, to investigate changes in genes and proteins expressed in the testis throughout spermatogenesis in F0 males by using a combination of transcriptomic and proteomic approaches and, second, to further compare the testis proteome between late spermatogenic stages of F0 and F1 fish to identify potential indicators of hampered reproductive performance in F1 fish. We identified approximately 400 genes and 49 proteins that are differentially expressed during the progression of spermatogenesis and that participate in processes such as transcriptional activation, the ubiquitin–proteasome system, sperm maturation and motility or cytoskeletal remodeling. Interestingly, a number of these proteins differed in abundance between F0 and F1 fish, pointing toward alterations in cytoskeleton, sperm motility, the ubiquitin–proteasome system and the redox state during spermiogenesis as possible causes for the decreased fertility of F1 fish.
Descripción : 17 pages, 7 figures, 2 tables.
Versión del editor: http://dx.doi.org/10.1002/pmic.201000296
URI : http://hdl.handle.net/10261/53298
ISSN: 1615-9853
DOI: 10.1002/pmic.201000296
Citación : Proteomics 11(11): 2195-2211 (2011)
Appears in Collections:(IIBB) Artículos
(CRAG) Artículos
(ICM) Artículos

Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.