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Title

Antibiotic resistance free plasmid DNA expressing LACK protein leads towards a protective Th1 response against Leishmania infantum infection

AuthorsRamos, Irene; Alonso, Ana ; Peris, Ana; Marcen, Jose Miguel; Abengozar, M. A. ; Alcolea, Pedro J. ; Castillo Hernández, Juan Antonio; Larraga, Vicente
KeywordsLeishmania infantum
Canine visceral leishmaniasis
LACK
Antibiotic resistance free DNA vaccine
Modified vaccinia virus Ankara
Issue Date12-Nov-2009
PublisherElsevier
CitationVaccine 27(48):6695-6703(2009)
AbstractCanine visceral leishmaniasis is a serious public health concern in the Mediterranean basin since dogs are the main Leishmania infantum reservoir. However, there is not a vaccination method in veterinary use in this area, and therefore the development of a vaccine against this parasite is essential for the possible control of the disease. Previous reports have shown the efficacy of heterologous prime-boost vaccination with the pCIneo plasmid and the poxvirus VV (both Western Reserve and MVA strains) expressing L. infantum LACK antigen against canine leishmaniasis. As pCIneo-LACK plasmid contains antibiotic resistance genes, its use as a profilactic method is not recommended. Hence, the antibiotic resistance gene free pORT-LACK plasmid is a more suitable tool for its use as a vaccine. Here we report the protective and immunostimulatory effect of the prime-boost pORT-LACK/MVA-LACK vaccination tested in a canine experimental model. Vaccination induced a reduction in clinical signs and in parasite burden in the liver, an induction of the Leishmania-specific T cell activation, as well as an increase of the expression of Th1 type cytokines in PBMC and target organs
Description9 páginas, 6 figuras, 2 tablas -- PAGS nros. 6695-6703
Publisher version (URL)http://dx.doi.org/10.1016/j.vaccine.2009.08.091
URIhttp://hdl.handle.net/10261/53216
DOI10.1016/j.vaccine.2009.08.091
ISSN0264-410X
Appears in Collections:(CIB) Artículos
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