English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/53059
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

Three dimensional confocal and electron microscopy imaging define the dynamics and mechanisms of diploidisation at early stages of barley microspore-derived embryogenesis

AutorGonzález-Melendi, Pablo ; Ramírez, Carmen; Testillano, P.S. ; Kumlehn, Jochen; Risueño, María Carmen
Palabras claveAndrogenesis
Electron microscopy
Genome doubling
Hordeum
Nuclear fusion
3D-confocal imaging
Fecha de publicaciónsep-2005
EditorSpringer
CitaciónPlanta,222(1):47-57(2005)
ResumenIn order to determine the timing and mechanisms of the spontaneous diploidisation throughout microspore-derived embryogenesis in barley, we have estimated the ploidy level of individual nuclei within young pro-embryos, from the first androgenetic division up to multinuclear structures still surounded by the exine. Our methodological approach was based on the measure of the intensity of fluorescence after 4,6-Diamidino-2-phenylindole dihydrochloride staining, nuclear size and number of nucleoli in the confocal microscope. This method avoids the overlapping of the fluorescence signal in multinuclear pro-embryos, which cannot be studied using cytophotometer methods based on other types of fluorescence microscopes. The identification of haploid and diploid nuclei enabled us to determine the timing of diploidisation at early stages throughout androgenetic development. We found that diploidisation is an ongoing process that can start after the first embyogenic division and continues in multinuclear pro-embryos. Reconstruction of 3D-images of entire pro-embryos and the observation of cross and longitudinal sections across stacks of optical sections, together with correlative light and electron microscopy, provided evidences of nuclear fusion as the main mechanism of diploidisation
Descripción11 páginas, 6 figuras, 2 tablas -- PAGS nros. 47-57
Versión del editorhttp://dx.doi.org/10.1007/s00425-005-1515-7
URIhttp://hdl.handle.net/10261/53059
DOI10.1007/s00425-005-1515-7
ISSN0032-0935
E-ISSN1432-2048
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
restringido.pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.