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Open Access item Expression of digestive enzyme precursors under different feeding conditions in sparus aurata larvae

Authors:Sánchez-Amaya, María Isabel
Yúfera, Manuel
Martínez-Rodríguez, Gonzalo
Issue Date:2009
Publisher:European Aquaculture Society
Citation:European Aquaculture Society. Special Publication (38) : 388-391 (2009)
Abstract:The onset of feeding is a crucial step during the development of marine fish lar-vae. As with most marine species, the digestive tract is not totally mature at mouth opening, but it develops over the whole larval period (Sánchez-Amaya et al., 2007). The digestive capacity during early development of the digestive sys-tem is provided by the pancreatic enzymes (proteases, lipases and glucosidases) in conjunction with alkaline proteolitic enzymes secreted by the intestine, which are important prior to stomach development. Most studies examining the ontog-eny of fish digestive system has been performed in hatchery-reared species, in order to achieve an understanding of their nutritional needs. These studies have been focused in designing formulated microdiets for replacing live prey during the first days of feeding (Kolkovski, 2001). Adequate nutrition, one of the main factors affecting larval survival, depends on the effective ingestion, digestion and assimilation of diets containing the re-quired essential nutrients. Digestion is a key process in animal metabolism, since it determines the availability of nutrients needed for all biological functions. The study of digestive enzymes expression is a useful tool when studying the nutri-tional condition and adaptation of the organism to dietary change. In the present study, full length cDNAs from ¿-amylase, trysinogen and bile ac-tivated lipase (BAL) have been cloned and sequenced in gilthead seabream, and their expression analyzed by in situ hybridization and Q-RT-PCR during the first weeks of larval development and under different feeding conditions (starvation, live prey and inert microdiets). The aim of this work was to study the expression onset and ontogeny of these enzymes in Sparus aurata in order to better under-stand the sequence of events occurring during the alkaline digestion in larval development and to gain insights into the digestive physiology from hatching stage onwards.
Description:5th Fish and Shellfish Larviculture Symposium. Larvi 2009. September 7-10, 2009. Ghent University, Belgium.
Appears in Collections:(ICMAN) Comunicaciones congresos

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