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Title

Genetic markers for doubled haploid response in barley

AuthorsChen, Xi-Wen; Cistué Sola, Luis ; Muñoz-Amatriaín, María ; Sanz Madoz, José Miguel ; Romagosa, Ignacio; Castillo Alonso, Ana María ; Vallés Brau, María Pilar
KeywordsAnther culture
barley
doubled haploid (DH)
microspore embryogenesis
QTLs
Issue DateDec-2007
PublisherSpringer
CitationEuphytica, Volume 158, Number 3, pages 287-294
AbstractIn order to analyse the genetic control of anther culture response in barley, a doubled haploid (DH) population from the cross between a medium responsive cultivar ‘Dobla’ and the model cultivar ‘Igri’ was produced. A linkage map was constructed with 91 markers. A sub-population of 41 lines was characterised for different components of the anther culture response, and was used for quantitative trait loci (QTL) analysis. The vrs1 locus region on chromosome 2H, which determines inflorescence row type, was coincident with the largest putative QTL for number of embryos (nEMB) and albino plants. A region of chromosome 6H was associated with QTLs for nEMB and green plants. QTLs for number and percentage of green plants were located on the long arm of chromosome 5H. Therefore, new QTLs for main components of barley anther culture response were identified on chromosomes 2H, 5H and 6H, indicating that anther culture response in barley could be controlled by relative few genes of large effect. This work is a useful step towards the identification of new regions on the barley genome that could be associated with fundamental biological process implicated in the anther culture response.
DescriptionThe original publication is available at http://www.springerlink.com/content/lg4126j81q8x15m2/fulltext.pdf
URIhttp://hdl.handle.net/10261/5212
DOI10.1007/s10681-006-9310-5
ISSN0014-2336
E-ISSN1573-5060
Appears in Collections:(EEAD) Artículos
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