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Title

The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E

AuthorsChittka, Alexandra; Pérez González, Pilar
Keywordsp75NTR
Cell cycle
HDAC
Transcriptional repressor
PR/SET domains
Issue DateMar-2004
PublisherRockefeller University Press
CitationJournal of Cell Biology 164(7): 985–996 (2004)
AbstractSchwann cell factor 1 (SC1), a p75 neurotrophin receptor–interacting protein, is a member of the positive regulatory/suppressor of variegation, enhancer of zeste, trithorax (PR/SET) domain-containing zinc finger protein family, and it has been shown to be regulated by serum and neurotrophins. SC1 shows a differential cytoplasmic and nuclear distribution, and its presence in the nucleus correlates strongly with the absence of bromodeoxyuridine (BrdU) in these nuclei. Here, we investigated potential transcriptional activities of SC1 and analyzed the function of its various domains. We show that SC1 acts as a transcriptional repressor when it is tethered to Gal4 DNA-binding domain. The repressive activity requires a trichostatin A–sensitive histone deacetylase (HDAC) activity, and SC1 is found in a complex with HDACs 1, 2, and 3. Transcriptional repression exerted by SC1 requires the presence of its zinc finger domains and the PR domain. Additionally, these two domains are involved in the efficient block of BrdU incorporation by SC1. The zinc finger domains are also necessary to direct SC1's nuclear localization. Lastly, SC1 represses the promoter of a promitotic gene, cyclin E, suggesting a mechanism for how growth arrest is regulated by SC1.
Description12 páginas, 9 figuras.-- et al.
Publisher version (URL)http://dx.doi.org/10.1083/jcb.200301106
URIhttp://hdl.handle.net/10261/5175
DOI10.1083/jcb.200301106
ISSN0021-9525
Appears in Collections:(IMB) Artículos
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