English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/5175
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E

AuthorsChittka, Alexandra; Pérez González, Pilar
Cell cycle
Transcriptional repressor
PR/SET domains
Issue DateMar-2004
PublisherRockefeller University Press
CitationJournal of Cell Biology 164(7): 985–996 (2004)
AbstractSchwann cell factor 1 (SC1), a p75 neurotrophin receptor–interacting protein, is a member of the positive regulatory/suppressor of variegation, enhancer of zeste, trithorax (PR/SET) domain-containing zinc finger protein family, and it has been shown to be regulated by serum and neurotrophins. SC1 shows a differential cytoplasmic and nuclear distribution, and its presence in the nucleus correlates strongly with the absence of bromodeoxyuridine (BrdU) in these nuclei. Here, we investigated potential transcriptional activities of SC1 and analyzed the function of its various domains. We show that SC1 acts as a transcriptional repressor when it is tethered to Gal4 DNA-binding domain. The repressive activity requires a trichostatin A–sensitive histone deacetylase (HDAC) activity, and SC1 is found in a complex with HDACs 1, 2, and 3. Transcriptional repression exerted by SC1 requires the presence of its zinc finger domains and the PR domain. Additionally, these two domains are involved in the efficient block of BrdU incorporation by SC1. The zinc finger domains are also necessary to direct SC1's nuclear localization. Lastly, SC1 represses the promoter of a promitotic gene, cyclin E, suggesting a mechanism for how growth arrest is regulated by SC1.
Description12 páginas, 9 figuras.-- et al.
Publisher version (URL)http://dx.doi.org/10.1083/jcb.200301106
Appears in Collections:(IMB) Artículos
Files in This Item:
File Description SizeFormat 
cell.pdf3,37 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.