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Title: Feasibility and application of liquid-liquid extraction combined with gas chromatography-mass spectrometry for the analysis of phenolic acids from grape polyphenols degraded by human faecal microbiota
Authors: Muñoz-González, Carolina ; Moreno-Arribas, M. Victoria ; Rodríguez-Bencomo, Juan José ; Cueva, Carolina ; Martín-Álvarez, Pedro J. ; Bartolomé, Begoña ; Pozo-Bayón, Mª Ángeles
Issue Date: 2012
Publisher: Elsevier
Citation: Food Chemistry 133(2): 526-535 (2012)
Abstract: In this study the feasibility of a LLE-GC-EI-MS method for the analysis of 43 phenolic acids belonging to different chemical structure families which have been described in the literature as microbial-derived metabolites after consumption of dietary polyphenols was proved. In addition, the method was applied for the characterisation of phenolic metabolites resulting from the incubation, in anaerobic conditions, of a commercial grape seed extract (GSE) and their corresponding flavan-3-ol monomeric (GSE-M) and oligomeric (GSE-O) fractions with human faeces from healthy volunteers (n = 3). The method showed average values of repeatability and reproducibility of 5.0% and 6.3%, respectively, adequate and low detection (1.8-30.8 μg L -1) and quantification limits (6.0-102.8 μg L -1) and good recovery values (95%, as average value). A total of 27 phenolic acids were identified in the faecal solutions after incubation with the grape seed extracts. In general, faecal samples incubated with GSE and GSE-M (monomeric fraction) yield a higher formation of phenolic acids compared to the samples incubated with the oligomer fraction (GSE-O). © 2012 Elsevier Ltd. All rights reserved.
Publisher version (URL): http://dx.doi.org/10.1016/j.foodchem.2012.01.020
URI: http://hdl.handle.net/10261/51707
DOI: 10.1016/j.foodchem.2012.01.020
Identifiers: doi: 10.1016/j.foodchem.2012.01.020
issn: 0308-8146
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