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http://hdl.handle.net/10261/51337
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Chiesa, Marco | es_ES |
dc.contributor.author | Cardenas, P. P. | es_ES |
dc.contributor.author | Otón, Francisco | es_ES |
dc.contributor.author | Martínez Rodrigo, Javier | es_ES |
dc.contributor.author | Mas Torrent, Marta | es_ES |
dc.contributor.author | García-Pérez, Fernando | es_ES |
dc.contributor.author | Alonso, Juan Carlos | es_ES |
dc.contributor.author | Rovira, Concepció | es_ES |
dc.contributor.author | García García, Ricardo | es_ES |
dc.date.accessioned | 2012-06-12T09:03:32Z | - |
dc.date.available | 2012-06-12T09:03:32Z | - |
dc.date.issued | 2012 | - |
dc.identifier | issn: 1530-6984 | - |
dc.identifier.citation | Nano Letters 12(3): 1275-1281 (2012) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/51337 | - |
dc.description.abstract | A silicon nanowire-based biosensor has been designed and applied for label-free and ultrasensitive detection of the early stage of recombinational DNA repair by RecA protein. Silicon nanowires transistors were fabricated by atomic force microscopy nanolithography and integrated into a microfluidic environment. The sensor operates by measuring the changes in the resistance of the nanowire as the biomolecular reactions proceed. We show that the nanoelectronic sensor can detect and differentiate several steps in the binding of RecA to a single-stranded DNA filament taking place on the nanowire-aqueous interface. We report relative changes in the resistance of 3.5% which are related to the interaction of 250 RecA·single-stranded DNA complexes. Spectroscopy data confirm the presence of the protein-DNA complexes on the functionalized silicon surfaces. © 2012 American Chemical Society. | - |
dc.description.sponsorship | This work was made possible by the CSIC through its interdisciplinary collaborative program (PIF08-008). We also acknowledge the partial financial support from the Ministerio de Ciencia, Investigación e Innovación (MAT2009-08650 and CTQ2010-195011/BQU), the Generalitat de Catalunya (2009SGR00516), and the CIBER de Bioingeniera, Biomateriales y Nanomedicina (CIBER-BBN), promoted by ISCIII, Spain; M.C. and F.O. acknowledge the program “Juan de la Cierva” (MICINN). | - |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.rights | closedAccess | - |
dc.title | Detection of the early stage of recombinational DNA repair by silicon nanowire transistors | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1021/nl2037547 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1021/nl2037547 | - |
dc.date.updated | 2012-06-12T09:03:32Z | - |
dc.description.version | Peer Reviewed | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Generalitat de Catalunya | - |
dc.contributor.funder | Instituto de Salud Carlos III | - |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002809 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (CNB) Artículos (ICMAB) Artículos (IMN-CNM) Artículos |
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