Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/50974
Título : Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor
Autor : Peña, Álvaro, Gewartowski, Kamil, Mroczek, Seweryn, Cuéllar, Jorge, Szykowska, Aleksandra, Prokop, Andrzej, Czarnocki-Cieciura, Mariusz, Piwowarski, Jan, Tous, Cristina, Aguilera, Andrés, Carrascosa, José L., Valpuesta, José María, Dziembowski, Andrzej
Palabras clave : Electron microscopy
mRNA export
mRNP quality control
THO complex
TREX complex
Fecha de publicación : 7-Feb-2012
Editor: Nature Publishing Group
Resumen: The THO complex is a key factor in co-transcriptional formation of export-competent messenger ribonucleoprotein particles, yet its structure and mechanism of chromatin recruitment remain unknown. In yeast, this complex has been described as a heterotetramer (Tho2, Hpr1, Mft1, and Thp2) that interacts with Tex1 and mRNA export factors Sub2 and Yra1 to form the TRanscription EXport (TREX) complex. In this study, we purified yeast THO and found Tex1 to be part of its core. We determined the three-dimensional structures of five-subunit THO complex by electron microscopy and located the positions of Tex1, Hpr1, and Tho2 C-terminus using various labelling techniques. In the case of Tex1, a β-propeller protein, we have generated an atomic model which docks into the corresponding part of the THO complex envelope. Furthermore, we show that THO directly interacts with nucleic acids through the unfolded C-terminal region of Tho2, whose removal reduces THO recruitment to active chromatin leading to mRNA biogenesis defects. In summary, this study describes the THO architecture, the structural basis for its chromatin targeting, and highlights the importance of unfolded regions of eukaryotic proteins. © 2012 European Molecular Biology Organization.
Versión del editor: http://dx.doi.org/10.1038/emboj.2012.10
URI : http://hdl.handle.net/10261/50974
Identificadores: doi: 10.1038/emboj.2012.10
issn: 0261-4189
Citación : EMBO Journal 31(6): 1605-1616 (2012)
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