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Molecular and functional characterization of a Schistosoma bovis annexin: Fibrinolytic and anticoagulant activity

AuthorsTorre Escudero, Eduardo de la ; Manzano Román, Raúl ; Siles Lucas, Mar ; Pérez Sánchez, Ricardo ; Moyano, J. C.; Barrera, I.; Oleaga, Ana
Schistosoma bovis
Anticoagulant activity
Issue Date2012
CitationVeterinary Parasitology 184 (1) : 25-36 (2012)
AbstractAnnexins belong to an evolutionarily conserved multigene family of proteins expressed throughout the animal and plant kingdoms. Although they are soluble cytosolic proteins that lack signal sequences, they have also been detected in extracellular fluids and have been associated with cell surface membranes, where they could be involved in anti-haemostatic and anti-inflammatory functions. Schistosome annexins have been identified on the parasite’s tegument surface and excretory/secretory products, but their functions are still unknown. Here we report the cloning, sequencing, in silico analysis, and functional characterization of a Schistosoma bovis annexin. The predicted protein has typical annexin secondary and tertiary structures. Bioassays with the recombinant protein revealed that the protein is biologically active in vitro, showing fibrinolytic and anticoagulant properties. Finally, the expression of the native protein on the tegument surface of S. bovis schistosomula and adult worms is demonstrated, revealing the possibility of exposure to the host’s immune system and thus offering a potential vaccine target for the control of schistosomiasis in ruminants.
Description31 páginas, 7 figuras. -- The definitive version is available at http://www.elsevier.com
Publisher version (URL)http://dx.doi.org/10.1016/j.vetpar.2011.08.013
Appears in Collections:(IRNASA) Artículos
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