Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/50398
Share/Impact:
Título : Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
Autor : Gestal, C., Costa, M. M., Figueras, Antonio, Novoa, Beatriz
Palabras clave : Antimicrobial peptides
Clam
Immune response
Myticin
Mytilin
Ruditapes decussatus
SSH
Fecha de publicación : 2007
Editor: Elsevier
Citación : Gene 406(1-2): 134-143 (2007)
Resumen: Suppression subtractive hybridization was used to identify differentiallyexpressedgenes in hemocytes from carpet-shell clam Ruditapes decussatus stimulated with a mixture of dead bacterial strains. Putative function could be assigned to 100 of the 253 sequenced cDNAs. Based on sequence homologies, 3.16% of the total identified genes were possibly related to immune functions. Clam myticin isoforms 1, 2 and 3, and clam mytilin, with similarity with myticins and mytilins previously reported on Mytilus galloprovincialis were identified and characterized for the first time in clams. The analysis of their expression levels by quantitative PCR showed that they were induced by bacterial challenge. The results obtained in this work could be the first step leading to the understanding of molecular mechanisms by which these economically important marine bivalves respond to pathogens
Descripción : 10 páginas, 6 figuras, 2 tablas
Versión del editor: http://dx.doi.org/10.1016/j.gene.2007.07.030
URI : http://hdl.handle.net/10261/50398
ISSN: 0378-1119
DOI: 10.1016/j.gene.2007.07.030
Citación : Gene 406(1-2): 134-143 (2007)
Appears in Collections:(IIM) Artículos

Files in This Item:
File Description SizeFormat 
Analysis_differentially_expressed_genes.pdf492,88 kBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.