Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/50313
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Relationship between lunasin's sequence and its inhibitory activity of histones H3 and H4 acetylation |
Autor: | Hernández-Ledesma, Blanca CSIC ORCID ; Hsieh, Chia-Chien; Lumen, Ben O. de | Palabras clave: | Breast cancer cells Cell proliferation Histone acetylation Lunasin Protein biomarkers |
Fecha de publicación: | jul-2011 | Editor: | Wiley-VCH | Citación: | Molecular Nutrition and Food Research 55(7): 989-998 (2011) | Resumen: | [Scope]: Dysfunction of histone acetyltransferases (HATs) or histone deacetylases (HDACs) involved in histones acetylation has been associated with cancer. Inhibitors of these enzymes are becoming potential targets for new therapies. [Methods and Results]: This study reports by Western-Blot analysis, that peptide lunasin is mainly an in vitro inhibitor of histone H4 acetylation by P300/cAMP-response element-binding protein (CBP)-associated factor (PCAF), with IC50 values dependent on the lysine position sensitive to be acetylated (0.83 μM (H4-Lys 8), 1.27 μM (H4-Lys 12) and 0.40 μM (H4-Lys 5, 8, 12, 16)). Lunasin is also capable of inhibiting H3 acetylation (IC50 of 5.91 μM (H3-Lys 9) and 7.81 μM (H3-Lys 9, 14)). Studies on structure-activity relationship establish that lunasin's sequence are essential for inhibiting H4 acetylation whereas poly-D sequence is the main active sequence responsible for H3 acetylation inhibition. Lunasin also inhibits H3 and H4 acetylation and cell proliferation (IC50 of 181μM) in breast cancer MDA-MB-231 cells. Moreover, this peptide decreases expression of cyclins and cyclin dependent kinases-4 and -6, implicated in cell cycle pathways. [Conclusion]: Results from this study demonstrates lunasin's role as modulator of histone acetylation and protein expression that might contribute on its chemopreventive properties against breast cancer. | Descripción: | 10 páginas, 5 figuras, 1 tabla. | Versión del editor: | http://dx.doi.org/10.1002/mnfr.201000632 | URI: | http://hdl.handle.net/10261/50313 | DOI: | 10.1002/mnfr.201000632 | ISSN: | 1613-4125 | E-ISSN: | 1613-4133 |
Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
49
checked on 27-mar-2024
WEB OF SCIENCETM
Citations
39
checked on 29-feb-2024
Page view(s)
313
checked on 18-abr-2024
Download(s)
316
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.