DSpace

Digital.CSIC > Ciencias Agrarias > Instituto de Ciencias Agrarias (ICA) > (ICA) Artículos >

Share

EndNote

Impact

Closed Access item Quantitative estimation of plum pox virus targets acquired and transmitted by a single Myzus persicae

Authors:Moreno, Aránzazu
Fereres, Alberto
Cambra, Mariano
Keywords:Myzus persicae, PPV, Virology, RT-PCR, RNA
Issue Date:2009
Publisher:Springer
Citation:Archives of Virology 154: 1391-1399 (2009)
Abstract:The viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (PPV). A combination of electrical penetration graph and TaqMan real-time RT-PCR techniques was used to establish the average number of PPV RNA targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. This number of PPV targets is responsible for a systemic infection of 20% on the inoculated receptor plants. No significant differences were found between the number of PPV RNA targets acquired after one and after five intracellular punctures (pd), but the frequency of infected receptor plants was higher after 5 pd. The percentage of PPV-positive leaf discs after just 1 pd of inoculation probe (28%/4,603 targets) was lower than after 5 pd (45.8%/135 9 106 targets). The methodology employed could be easily extended to other virus–vector–host combinations to improve the accuracy of models used in virus epidemiology.
Description:9 páginas, ilustraciones y tablas estadísticas
Publisher version (URL):http://dx.doi.org/10.1007/s00705-009-0450-5
URI:http://hdl.handle.net/10261/50279
ISSN:0304-8608
???metadata.dc.identifier.doi???:10.1007/s00705-009-0450-5
Appears in Collections:(ICA) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.