English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/50144
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Identification of the first functional toxin-antitoxin system in streptomyces

AuthorsSevillano, Laura ; Díaz, Margarita ; Yamaguchi, Y.; Inouye, M.; Santamaría, Ramón I.
Issue Date2012
PublisherPublic Library of Science
CitationPLoS ONE 7(3): e32977 (2012)
AbstractToxin-antitoxin (TA) systems are widespread among the plasmids and genomes of bacteria and archaea. This work reports the first description of a functional TA system in Streptomyces that is identical in two species routinely used in the laboratory: Streptomyces lividans and S. coelicolor. The described system belongs to the YefM/YoeB family and has a considerable similarity to Escherichia coli YefM/YoeB (about 53% identity and 73% similarity). Lethal effect of the S. lividans putative toxin (YoeBsl) was observed when expressed alone in E. coli SC36 (MG1655 ΔyefM-yoeB). However, no toxicity was obtained when co-expression of the antitoxin and toxin (YefM/YoeBsl) was carried out. The toxic effect was also observed when the yoeBsl was cloned in multicopy in the wild-type S. lividans or in a single copy in a S. lividans mutant, in which this TA system had been deleted. The S. lividans YefM/YoeBsl complex, purified from E. coli, binds with high affinity to its own promoter region but not to other three random selected promoters from Streptomyces. In vivo experiments demonstrated that the expression of yoeBsl in E. coli blocks translation initiation processing mRNA at three bases downstream of the initiation codon after 2 minutes of induction. These results indicate that the mechanism of action is identical to that of YoeB from E. coli. © 2012 Sevillano et al.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0032977
URIhttp://hdl.handle.net/10261/50144
DOI10.1371/journal.pone.0032977
Identifiersdoi: 10.1371/journal.pone.0032977
issn: 1932-6203
Appears in Collections:(IBFG) Artículos
Files in This Item:
File Description SizeFormat 
Identification of the First Functional.pdf1,51 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.