English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49993
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The Toxin-Antitoxin proteins RelBE2Spn of Streptococcus pneumoniae: Characterization and association to their DNA target

AuthorsMoreno-Córdoba, Inmaculada; Diago-Navarro, Elizabeth ; Barendregt, Arjan; Heck, Albert J. R.; Alfonso, Carlos ; Díaz-Orejas, Ramón ; Nieto, Concha ; Espinosa, Manuel
KeywordsPneumococcal RelB and RelE proteins
Protein–protein interactions
Protein–DNA interactions
Native mass spectrometry
Analytical ultracentrifugation
Issue Date10-Apr-2012
PublisherWiley-Blackwell
CitationProteins. 2012 Jul;80(7):1834-46
AbstractThe chromosome of the pathogenic Gram-positive bacterium Streptococcus pneumoniae contains between six to 10 operons encoding toxin-antitoxin systems (TAS). TAS are widespread and redundant in bacteria and archaea and their role, albeit still obscure, may be related to important aspects of bacteria lifestyle like response to stress. One of the most abundant TAS is the relBE family, being present in the chromosome of many bacteria and archaea. Because of the high rates of morbility and mortality caused by S. pneumoniae, it has been interesting to gain knowledge on the pneumococcal TAS, among them the RelBE2Spn proteins. Here, we have analyzed the DNA binding capacity of the RelB2Spn antitoxin and the RelB2Spn-RelE2Spn proteins by band-shift assays. Thus, a DNA region encompassing the operator region of the proteins was identified. In addition, we have used analytical ultracentrifugation and native mass spectrometry to measure the oligomerization state of the antitoxin alone and the RelBE2Spn complex in solution bound or unbound to its DNA substrate. Using native mass spectrometry allowed us to unambiguously determine the stoichiometry of the RelB2Spn and of the RelBE2Spn complex alone or associated to its DNA target
Publisher version (URL)http://dx.doi.org/ 10.1002/prot.24081
URIhttp://hdl.handle.net/10261/49993
DOI10.1002/prot.24081
ISSN0887-3585
E-ISSN1097-0134
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.