Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/49731COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Nucleolar disruption ensures nuclear accumulation of p21 upon DNA damage |
Autor: | Tapia, Olga; Berciano, María T.; Lafarga, Miguel; Agell, Neus | Palabras clave: | DNA damage NPM/B23 |
Fecha de publicación: | 2010 | Editor: | Wiley-Blackwell | Citación: | Traffic 11(6): 743–755 (2010) | Resumen: | p21cip1 is a protein with a dual function in oncogenesis depending mainly on its intracellular localization: tumor suppressor in the nucleus and oncogenic in the cytoplasm. After DNA damage, p21cip1 increases and accumulates in the nucleus to ensure cell cycle arrest. We show here that the nuclear accumulation of p21cip1 is not only a consequence of its increased levels but to a DNA damage cellular response, which is ataxia telangiectasia and Rad3 related (ATR)/ataxia telangiectasia mutated (ATM) and p53 independent. Furthermore, after DNA damage, p21cip1 not only accumulates in the nucleoplasm but also in the disrupted nucleolus. Inside the nucleolus, it is found in spherical structures, which are not a protrusion of the nucleoplasm. The steady-state distribution of p21cip1 in the nucleolus resulted from a highly dynamic equilibrium between nucleoplasmic and nucleolar p21cip1 and correlated with the inhibition of p21cip1 nuclear export. Most interestingly, inhibition of ribosomal export after expressing a dominant-negative mutant of nucleophosmin induced p21cip1 accumulation in the nucleus and the nucleolus in the absence of DNA damage. This proved the existence of a nucleolar export route to the cytoplasm for p21cip1 in control conditions that would be inhibited upon DNA damage leading to nuclear and nucleolar accumulation of p21cip1. | Descripción: | et al. | Versión del editor: | http://dx.doi.org/10.1111/j.1600-0854.2010.01063.x | URI: | http://hdl.handle.net/10261/49731 | DOI: | 10.1111/j.1600-0854.2010.01063.x | ISSN: | 1398-9219 |
| Aparece en las colecciones: | (IBBTEC) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| accesoRestringido.pdf | 15,38 kB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
27
checked on 09-abr-2024
WEB OF SCIENCETM
Citations
24
checked on 29-feb-2024
Page view(s)
280
checked on 18-abr-2024
Download(s)
91
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.