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Título: | Wine yeast molecular typing using a simplified method for simultaneously extracting mtDNA, nuclear DNA and virus dsRNA |
Autor: | Maqueda, Matilde; Zamora, Emiliano; Rodríguez-Cousiño, Nieves CSIC ORCID; Ramírez, Manuel CSIC | Palabras clave: | Yeast typing Nucleic acid extraction mtDNA Nuclear DNA dsRNA viruses |
Fecha de publicación: | abr-2010 | Editor: | Elsevier | Citación: | Food Microbiology 27(2): 205-209 (2010) | Resumen: | Quick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains. We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA. It is simpler, cheaper, and faster than the previously reported methods. It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different methods for the separate extraction of each nucleic acid type. The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing. | Descripción: | 5 páginas, 4 figuras, 1 tabla. | Versión del editor: | http://dx.doi.org/10.1016/j.fm.2009.10.004 | URI: | http://hdl.handle.net/10261/49634 | DOI: | 10.1016/j.fm.2009.10.004 | ISSN: | 0740-0020 |
Aparece en las colecciones: | (IBFG) Artículos |
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