Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49632
Share/Export:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invite to open peer review
Title

8-Dehydrosterols induce v-atpase dysfunction in yeast. Protective effect of an alternative H+-PUMP

AuthorsHernández, Agustín CSIC ORCID; López-Lluch, Guillermo CSIC ORCID CVN ; Pérez-Castiñeira, J. R. CSIC ORCID ; Serrano-Bueno, Gloria CSIC ORCID ; Navas, Plácido CSIC ORCID; Serrano, Aurelio CSIC ORCID
KeywordsToxic sterol precursors
8-dehydrosterols
Conradi-Hünnermann-Happle syndrome
Saccharomyces cerevisiae proteins
Issue Date12-Dec-2011
PublisherSociedad Española de Biología Celular
CitationXIV Congresos de la Sociedad Española de Biología Celular (2011)
AbstractToxic sterol precursors, such as 8-dehydrosterols, are present in a wide range of biologically relevant situations, from human rare diseases such as Conradi-Hünnermann-Happle Syndrome to amine fungicide-treated fungi and crops. However, the molecular and cellular bases of their toxicity are still obscure. Here we show that 8-dehydrosterols affect vacuole acidification producing Vma--like phenotypes in Saccharomyces cerevisiae. Upon expression of a gene construct of the proton-pumping membrane-embedded pyrophosphatase AVP1 from the plant Arabidopsis thaliana as an alternative proton pump, erg2 -derived strains revert their Vma--like phenotypes and wild-type cells can proliferate in the presence of lethal concentrations of tridemorph. Also, endo- and exocytotic defects in erg2 mutants are alleviated by the presence of a plant H+-pyrophosphatase. Despite their effect on endo/exocytic traffic, 8-dehydrosterols do not induce endoplasmic reticulum stress or assembly defects on the V-ATPase. On the contrary, they exert their influence directly at the vacuole level by inducing PEP4-dependent proteolytic degradation of V0 components, like a subunit Vph1p. These results can help to understand the etiology of sterol-related diseases, the fungicidal effect of amine compounds and the tolerance to this type of compounds observed in certain field fungi isolates and, in general, in plants.
DescriptionResumen del póster presentado al XIV Congresos de la Sociedad Española de Biología Celular, celebrado en Málaga del 12 al 15 de diciembre de 2011.
URIhttp://hdl.handle.net/10261/49632
Appears in Collections:(IBVF) Comunicaciones congresos
(CABD) Comunicaciones congresos




Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

Page view(s)

305
checked on Mar 29, 2024

Download(s)

48
checked on Mar 29, 2024

Google ScholarTM

Check


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.