English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49511
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A TaqMan real-time PCR-based assay for the identification of Fasciola spp.

AuthorsAlasaad, Samer ; Soriguer, Ramón C. ; Abu-Madi, Marawan; El Behairy, Ahmed; Jowers, Michael J.; Díez-Baños, Pablo; Píriz, Ana ; Fickel, Joerns; Zhu, Xing-Quang
KeywordsReal time qPCRTaqMan probe
Parasite identification
Fasciola hepatica
Fasciola gigantica
The ‘intermediate’ Fasciola
Issue DateJun-2011
PublisherElsevier
CitationVeterinary Parasitology 179 (2011) 266–271
AbstractReal time quantitative PCR (qPCR) is one of the key technologies of the post-genome era, with clear advantages compared to normal end-point PCR. In this paper, we report the first qPCR-based assay for the identification of Fasciola spp. Based on sequences of the second internal transcribed spacers (ITS-2) of the ribosomal rRNA gene, we used a set of genus- specific primers for Fasciola ITS-2 amplification, and we designed species-specific internal TaqMan probes to identify F. hepatica and F. gigantica, as well as the hybrid ‘intermediate’ Fasciola. These primers and probes were used for the highly specific, sensitive, and simple identification of Fasciola species collected from different animal host from China, Spain, Niger and Egypt. The novel qPCR-based technique for the identification of Fasciola spp. may provide a useful tool for the epidemiological investigation of Fasciola infection, including their intermediate snail hosts
Publisher version (URL)http://dx.doi.org/10.1016/j.vetpar.2011.01.059
URIhttp://hdl.handle.net/10261/49511
DOI10.1016/j.vetpar.2011.01.059
Appears in Collections:(EBD) Artículos
Files in This Item:
File Description SizeFormat 
1-s2.0-s0304401711000938-main.doc906,5 kBMicrosoft WordView/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.