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Lipidomic approaches to the study of phospholipase A2-regulated phospholipid fatty acid incorporation and remodeling

AuthorsBalgoma, David ; Montero, Olimpio; Balboa, María A. ; Balsinde, Jesús
Phospholipase A2
Mass spectrometry
Arachidonic acid
Issue DateJun-2010
CitationBiochimie 92(6): 645-650 (2010)
AbstractThe distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunity and inflammation. Because availability of free AA constitutes a rate-limiting step in the generation of eicosanoids by mammalian cells, many studies have been devoted to characterize the processes of arachidonate liberation from phospholipids by phospholipase A2s and its re-incorporation and further remodeling back into phospholipids by acyltransferases and transacylases. These studies have traditionally been conducted by using radioactive precursors which do not allow the identification of the phospholipid molecular species involved in these processes. Nowadays, lipidomic approaches utilizing mass spectrometry provide a new frame for the analysis of unique phospholipid species involved in fatty acid release and phospholipid incorporation and remodeling. This review focuses on the mass spectrometry techniques applied to the study of phospholipid fatty acid trafficking and the recent advances that have been achieved by the use of this technique.
Description6 páginas, 2 figuras, 1 tabla.-- El pdf del artículo es la versión post-print.
Publisher version (URL)http://dx.doi.org/10.1016/j.biochi.2009.11.010
Appears in Collections:(IBGM) Artículos
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