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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49283
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dc.contributor.authorMuñoz, Alfonso-
dc.contributor.authorBannenberg, Gerard L.-
dc.contributor.authorMontero, Olimpio-
dc.date.accessioned2012-05-07T09:31:45Z-
dc.date.available2012-05-07T09:31:45Z-
dc.date.issued2011-01-
dc.identifier.citationJournal of Experimental Botany 62(1): 307-318 (2011)es_ES
dc.identifier.issn0022-0957-
dc.identifier.urihttp://hdl.handle.net/10261/49283-
dc.description12 páginas, 7 figuras, 2 tablas.-- El documento en word es la versión post-print.-- et al.es_ES
dc.description.abstractUreidoglycolate is an intermediate in the degradation of the ureides, allantoin and allantoate, found in many organisms. In some leguminous plant species these compounds are used to transport recently fixed nitrogen in the root nodules to the aerial parts of the plant. In the present study, it was demonstrated that purified ureidoglycolases from chickpea (Cicer arietinum) and French bean (Phaseolus vulgaris) do not produce glyoxylate, and can use phenylhydrazine as a substrate with Km values of 4.0 mM and 8.5 mM, respectively. Furthermore, these enzymes catalyse the transfer of the ureidoglycolyl group to phenylhydrazine to produce ureidoglycolyl phenylhydrazide, which degrades non-enzymatically to glyoxylate phenylhydrazone and urea. This supports their former classification as ureidoglycolate urea-lyases. The enzymatic reaction catalysed by the characterized ureidoglycolases uncovered here can be viewed as a novel type of phenylhydrazine ureidoglycolyl transferase. The implications of these findings for ureide metabolism in legume nitrogen metabolism are discussed.es_ES
dc.description.sponsorshipThis work was supported by the Ministerio de Ciencia e Innovación (AGL2009-11290), Consejería de Innovación, Ciencia y Empresa de la Junta de Andalucía (CVI1761), and Plan Andaluz de Investigación (CVI-115). G Bannenberg is a Ramón y Cajal fellow supported by the Centro Nacional de Biotecnología/CSIC and the Ministry of Science and Innovation, Spain.es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.rightsopenAccesses_ES
dc.subjectAllantoines_ES
dc.subjectAllantoatees_ES
dc.subjectCiceres_ES
dc.subjectNitrogen metabolismes_ES
dc.subjectPhaseoluses_ES
dc.subjectPhenylhydrazinees_ES
dc.subjectUreide degradationes_ES
dc.subjectUreidoglycolatees_ES
dc.titleAn alternative pathway for ureide usage in legumes: enzymatic formation of a ureidoglycolate adduct in Cicer arietinum and Phaseolus vulgarises_ES
dc.typeartículoes_ES
dc.identifier.doi10.1093/jxb/erq268-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1093/jxb/erq268es_ES
dc.identifier.e-issn1460-2431-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
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