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Closed Access item Identification of european hake species (Merluccius merluccius) using real-time PCR

Authors:Sánchez, Ana Cristina
Quinteiro, Javier
Rey Méndez, Manuel
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
Keywords:European hake, Species identification, Real-time PCR, Merluccius merluccius, Control region
Issue Date:2009
Publisher:American Chemical Society
Citation:Journal of Agricultural and Food Chemistry 57(9): 3397-3403 (2009)
Abstract:A rapid and precise method for identifying European hake (Merluccius merluccius) based on TaqMan technology is presented. The method can be applied to fresh, frozen, and processed fish products to detect the fraudulent or unintentional mislabeling of this species. Specific primers and a minor groove binding (MGB) TaqMan probe were designed for this purpose based on partial sequences of the mitochondrial DNA control region. Combinations of primers and probe concentrations that gave the lowest Ct value and the highest final fluorescence value were selected to carry out efficiency, specificity, and cross-reactivity assays. The method was successfully tested on 31 commercial hake samples. A Ct value of about 16 was obtained when Merluccius merluccius was present; however, the fluorescence signal was not detected most of the time (Ct value 40) or presented significantly higher Ct values (38.2 ± 0.96) for the nonhake species
Description:7 páginas, 3 figuras, 4 tablas
Publisher version (URL):http://dx.doi.org/10.1021/jf8036165
E-ISSNmetadata.dc.identifier.doi = DOI:1520-5118
Appears in Collections:(IIM) Artículos

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