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Título

Determination of conjugation rates on solid surfaces

AutorCampo, Irene del ; Ruiz, Raúl ; Cuevas, Ana ; Revilla, Carlos ; Vielva, Luis; Cruz, Fernando de la
Palabras claveBacterial conjugation
Surface mating
Flow-cytometer
GFP
Fecha de publicaciónmar-2012
EditorElsevier
CitaciónPlasmid 67(2): 174–182 (2012)
ResumenA cytometric method for the estimation of end-point conjugation rates is developed and adapted to surface conjugation. This method improves the through-put of conjugation assays based on replica-plating and results in less noisy experimental data. Although conjugation on solid surfaces deviates from ideal conditions in which cells are continuously mixed, results show that, within the limits of high initial population densities and short mating times, end-point estimates of the conjugation rates are robust measurements. They are independent of the donor/recipient ratios and, to some extent, of the sampling time. Remixing the mating population in the course of a conjugation experiment results in a boost in the frequency of transconjugants.
DescripciónA special issue on chromosome dynamics in memory of Prof. Kurt Nordstrom.
Versión del editorhttp://dx.doi.org/10.1016/j.plasmid.2012.01.008
URIhttp://hdl.handle.net/10261/48907
DOI10.1016/j.plasmid.2012.01.008
ISSN0147-619X
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