Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/48724
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and rapd analysis of genetic stability |
Autor: | Sánchez Fernández, M. Concepción CSIC ORCID; Martínez-Santiago, María Teresa CSIC ORCID; Vidal, Nieves; San José, M. Carmen; Valladares, Silvia CSIC ORCID; Viéitez Martín, Ana María CSIC | Palabras clave: | cryopreservation Genetic fidelity Oak plant regeneration somatic embryogenesis vitrification |
Fecha de publicación: | 2008 | Editor: | National Institutes of Health (U.S.). PubMed Central | Citación: | Cryoletters 29 (6) 493-504 (2008) | Resumen: | This study reports on the cryostorage of embryogenic lines derived from selected mature Quercus robur trees, following application of the PVS2-vitrification based procedure. In seven oak genotypes, embryo recovery levels ranging from 57-92% were obtained when 4-6 mg embryo clumps were precultured for 3 days on 0.3 M sucrose basal medium, treated with PVS2 solution for 60 min at 24ºC, and then immersed in liquid nitrogen (LN). Embryos of six out of seven lines were cryostored for one week and one year and used to evaluate cryopreservation tolerance, germination ability and to assess genetic fidelity by random amplified polymorphic DNA (RAPD) markers. There were no significant differences between the recovery frequencies of samples retrieved from LN after 1 week and 1 year of cryostorage. In five out of six lines, RAPD profiles of cryopreserved somatic embryos and regenerated plantlets were identical to those of the controls. Although polymorphisms were detected in only one cryostored embryo of one genotype, no genetic instability was found in the regenerated plantlets. This methodology appears to be suitable for long-term storage of this valuable germplasm, as the recovered plantlets were found to be genetically stable. | URI: | http://hdl.handle.net/10261/48724 |
Aparece en las colecciones: | (IIAG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Preservation of Quercus Robur. Sánchez, C. et al..pdf | 268,44 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
199
checked on 19-mar-2024
Download(s)
142
checked on 19-mar-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.