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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/48696
Title: Characterization and expression of a Pinus radiata putative ortholog to the Arabidopsis SHORT-ROOT gene
Authors: Solé, Alicia; Sánchez Fernández, M.ª Concepción; Vielba, Jesús; Valladares, Silvia; Abarca, Dolores; Díaz-Sala, Carmen
Keywords: adventitious rooting
GRAS genes
root meristem
vegetative propagation
Issue Date: 2008
Publisher: Heron Publishing
Citation: Tree Physiology 28 (11): 1629–1639 (2008)
Abstract: We characterized a Pinus radiata D. Don putative ortholog to the Arabidopsis thaliana (L.) Heynh. SHORT-ROOT gene (AtSHR) and analyzed its expression in different organs during vegetative development and in response to exogenous auxin during adventitious rooting. The predicted protein sequence contained domains characteristic of the GRAS protein family and showed a strong similarity to the SHORT-ROOT (SHR) proteins. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and in situ hybridization showed that the gene is predominantly expressed in roots, root primordia and in the cambial region of hypocotyl cuttings. Increased mRNA levels were observed, independently of the presence or absence of exogenous auxin, in the cambial region and rooting competent cells of hypocotyl cuttings within the first 24 h of adventitious rooting, before the activation of cell divisions and the organization of the adventitious root meristem. The expression pattern in organs and during adventitious rooting was similar to that of a Pinus radiata SCARECROW-LIKE (PrSCL1) gene, except that PrSCL1 is induced in response to exogenous auxin. Results suggest that the Pinus radiata SHORT-ROOT (PrSHR) gene has a role in root meristem formation and maintenance and in the cambial region of hypocotyl cuttings.
Publisher version (URL): http://dx.doi.org/10.1093/treephys/28.11.1629
URI: http://hdl.handle.net/10261/48696
DOI: 10.1093/treephys/28.11.1629
ISSN: 0829-318X
E-ISSN: 1758-4469
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