Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/48475
Share/Impact:
Título : Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus
Autor : Martí, Miguel, Trotonda, María Pilar, Tormo Más, María Ángeles, Vergara-Irigaray, Marta, Cheung, Ambrose L., Lasa, Íñigo, Penadés, José R.
Palabras clave : Staphylococcus aureus
Biofilm
Bap
SarA
σB
Extracellular proteases
Aureolysin
SspA
Fecha de publicación : Jan-2010
Editor: Elsevier
Citación : Microbes and Infection 12(1): 55-64 (2010)
Resumen: In Staphylococcus aureus, biofilm formation can be associated with the production of surface-anchored proteins, including Bap, SasG, FnBPs or Spa. By mutational analysis, and using a model strain in which biofilm formation was Bap-dependent, we found that σB was essential for protein-dependent biofilm development. Non-polar mutations of σB in genetically unrelated S. aureus strains lowered the Bap expression and completely impaired biofilm development. Although Northern blot analysis showed a slight reduction in bap transcription, we demonstrated that Aur and SspA, two proteases that are overexpressed in the sigB mutant strain and are capable of degrading Bap, inhibit biofilm formation. Interestingly, a double sigB-agr mutant, which showed a diminished capacity to express extracellular proteases, was able to restore biofilm formation. Since the vast majority of the S. aureus global regulators control the expression of the extracellular proteases, the results of this work demonstrate the existence of a new pathway controlling protein-mediated biofilm formation in which different global regulators modulate biofilm formation by controlling the expression of S. aureus extracellular proteases.
Descripción : 10 p., 5 figures, 1 table and bibliography
Versión del editor: http://dx.doi.org/10.1016/j.micinf.2009.10.005
URI : http://hdl.handle.net/10261/48475
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2009.10.005
Appears in Collections:(IDAB) Artículos

Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.