English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/48030
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 11 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Ligands of boron in Pisum sativum nodules are involved in regulation of oxygen concentration and rhizobial infection

Autor Reguera, María; Wimmer, Monika; Bustos-Sanmamed, Pilar ; Goldbach, Einer E.; Bolaños, Luis; Bonilla, Ildefonso
Palabras clave borate complexes
leghaemoglobin
legume AGP–extensin
legume rhizobin symbiosis
APX
Fecha de publicación jun-2010
EditorJohn Wiley & Sons
Citación Reguera M, Wimmer M, Bustos P, Goldbach EE, Bolaños L, Bonilla I. Ligands of boron in Pisum sativum nodules are involved in regulation of oxygen concentration and rhizobial infection. Plant Cell & Environment 33 (6): 1039-1048 (2010)
ResumenBoron (B) is an essential nutrient for N(2)-fixing legume-rhizobia symbioses, and the capacity of borate ions to bind and stabilize biomolecules is the basis of any B function. We used a borate-binding-specific resin and immunostaining techniques to identify B ligands important for the development of Pisum sativum- Rhizobium leguminosarum 3841 symbiotic nodules. arabinogalactan-extensin (AGPE), recognized by MAC 265 antibody, appeared heavily bound to the resin in extracts derived from B-sufficient, but not from Bdeficient nodules. MAC 265 stained the infection threads and the extracellular matrix of cortical cells involved in the oxygen diffusion barrier. In B-deprived nodules, immunolocalization of MAC 265 antigens was significantly reduced. Leghaemoglobin (Lb) concentration largely decreased in B-deficient nodules. The absence of MAC 203 antigens in B-deficient nodules suggests a high internal oxygen concentration, as this antibody detects an epitope on the lipopolysaccharide (LPS) of bacteroids typically expressed in microaerobically grown R. leguminosarum 3841. However, B-deprived nodules did not accumulate oxidized lipids and proteins, and revealed a decrease in the activity of the major antioxidant enzyme ascorbate peroxidase (APX). Therefore, B deficiency reduced the stability of nodule macromolecules important for rhizobial infection, and for regulation of oxygen concentration, resulting in non-functional nodules, but did not appear to induce oxidative damage in low-B nodules.
Descripción 16 Pags., 7 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3040
Versión del editorhttp://dx.doi.org/10.1111/j.1365-3040.2010.02125.x
URI http://hdl.handle.net/10261/48030
DOI10.1111/j.1365-3040.2010.02125.x
ISSN0140-7791
E-ISSN1365-3040
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BustosP_PlantCellEnv_2010.pdf1,39 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.