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Comparison of diagnostic techniques to detect the clam pathogen Perkinsus olseni

AuthorsBalseiro, P. ; Montes, Jaime; Fernández Conchas, Ramón; Novoa, Beatriz ; Figueras Huerta, Antonio
Ray’s fluid thioglycollate medium
Issue Date2010
PublisherInter Research
CitationDiseases of Aquatic Organisms 90(2): 143-151 (2010)
AbstractPerkinsosis in clams in Galicia (NW Spain) is caused by the protozoan parasite Perkinsus olseni Lester & Davis, 1981. We used 5 clam species of commercial interest cultured in Galicia (Ruditapes decussatus, R. philippinarum, Venerupis pullastra, V. rhomboides, and Donax trunculus) to compare various P. olseni diagnostic techniques. Results of a nested PCR assay for the diagnosis of P. olseni were compared to those obtained using 2 classical methods of diagnosis proposed by the World Organisation for Animal Health (OIE), viz. histology and incubation in Ray’s fluid thioglycollate medium (RFTM). Moreover, the same samples were analyzed by 2 separate research groups. The results obtained by PCR showed high sensitivity and good correlation between research groups. In addition, this method is faster than histopathology and incubation on RFTM and less expensive than histopathology. Moreover, nested PCR requires less specialized training for technicians than histology. Histopathology also showed high specificity and a good correlation between research groups. Results from incubation on RFTM suggest that this method could give divergent results between research groups, particularly in the case of low levels of infection, but it is nevertheless useful for disease-monitoring purposes. PCR is appropriate for rapidly screening large numbers of clams.
Description9 tablas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.3354/dao02194
Appears in Collections:(IIM) Artículos
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