Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/47951
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Título : Regulation of CC Ligand 5/RANTES by Acid Sphingomyelinase and Acid Ceramidase
Autor : Jenkins, Russell W., Clarke, Christopher J., Canals, Daniel, Snider, Ashley J., Gault, Christopher R., Heffernan-Stroud, Linda, Wu, Bill X., Simbari, Fabio, Roddy, Patrick, Kitatani, Kazuyuki, Obeid, Lina M., Hannun, Yusuf A.
Palabras clave : Chemokines
Glycoprotein Secretion
Inflammation
Lysosomes
Sphingolipid
Acid Ceramidase
Acid Sphingomyelinase
Fecha de publicación : 2011
Editor: American Society for Biochemistry and Molecular Biology
Citación : Journal of Biological Chemistry
Resumen: Acid sphingomyelinase (aSMase) generates the bioactive lipid ceramide (Cer) from hydrolysis of sphingomyelin (SM). However, its precise roles in regulating specific sphingolipid-mediated biological processes remain ill defined. Interestingly, the aSMase gene gives rise to two distinct enzymes, lysosomal sphingomyelinase (L-SMase) and secretory sphingomyelinase (S-SMase) via alternative trafficking of a shared protein precursor. Previously, our laboratory identified Ser508 as a crucial residue for the constitutive and regulated secretion of S-SMase in response to inflammatory cytokines, and demonstrated a role for S-SMase in formation of select cellular Cer species (Jenkins, R. W., Canals, D., Idkowiak-Baldys, J., Simbari, F., Roddy, P., Perry, D. M., Kitatani, K., Luberto, C., and Hannun, Y. A. (2010) J. Biol. Chem. 285, 35706–35718). In the present study using a chemokine/cytokine screen, we identified the chemokine CCL5 (formerly known as RANTES) as a candidate-specific downstream target for aSMase. Regulation of CCL5 by aSMase was subsequently validated using both loss-of-function and gain-of-function models indicating that aSMase is both necessary and sufficient for CCL5 production. Interestingly, cells deficient in acid ceramidase (aCDase) also exhibited defects in CCL5 induction, whereas cells deficient in sphingosine kinase-1 and -2 exhibited higher levels of CCL5, suggesting that sphingosine and not sphingosine 1-phosphate (S1P) is responsible for the positive signal to CCL5. Consistent with this, co-expression of aSMase and aCDase was sufficient to strongly induce CCL5. Taken together, these data identify a novel role for aSMase (particularly S-SMase) in chemokine elaboration by pro-inflammatory cytokines and highlight a novel and shared function for aSMase and aCDase.
Versión del editor: http://dx.doi.org/10.1074/jbc.M110.163378
URI : http://hdl.handle.net/10261/47951
ISSN: 0021-9258
DOI: 10.1074/jbc.M110.163378
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