Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/47803
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Sánchez Martínez, Elisa | - |
dc.contributor.author | Rubio, V. C. | - |
dc.contributor.author | Cerdá-Reverter, José Miguel | - |
dc.date.accessioned | 2012-04-02T10:37:14Z | - |
dc.date.available | 2012-04-02T10:37:14Z | - |
dc.date.issued | 2009-12 | - |
dc.identifier.citation | Journal of Experimental Biology 212(23): 3901-3910 (2009) | es_ES |
dc.identifier.issn | 0022-0949 | - |
dc.identifier.uri | http://hdl.handle.net/10261/47803 | - |
dc.description | 10 p., 8 figures and bibliography | es_ES |
dc.description.abstract | The melanocortin 5 receptor (MC5R) plays a key role in the regulation of exocrine secretion in mammalian species. This receptor has also been characterized in some fish species but its function is unknown. We report the molecular and pharmacological characterization, as well as the tissue expression pattern, of sea bass MC5R. Cloning of five active alleles showing different levels of sensitivity to endogenous melanocortin and one non-functional allele demonstrate the allelic complexity of the MC5R locus. The sea bass receptor was activated by all the melanocortins tested, with ACTH and desacetyl-MSH and -MSH showing the lowest efficiency. The acetylation of the MSH isoforms seems to be critical for the effectiveness of the agonist. Agouti-related protein had no effect on basal or agonist-stimulated activation of the receptor. SbMC5R was mainly expressed in the brain but lower expression levels were found in several peripheral tissues, including liver. Progressive fasting did not induce up- or downregulation of hypothalamic MC5R expression, suggesting that central MC5R is not involved in the regulation of food intake in the sea bass. MTII, a sbMC5R agonist, stimulated hepatic lipolysis in vitro, measured as free fatty acid release into the culture medium after melanocortin agonist exposure of liver fragments, suggesting that MC5R is involved in the regulation of hepatic lipid metabolism. Taken together, the data suggest that different allelic combinations may confer differential sensitivity to endogenous melanocortin in tissues where MC5R is expressed and, by extension, in hepatic lipid metabolism. | es_ES |
dc.description.sponsorship | Funded by AGL2004-08137-C04-04; AGL2007-65744-C03-02; CSD 2007-00002 from the Spanish Science and Innovation Ministry to J. M. C.-R. V. C. R. was recipient of a "Juan de la Cierva" research contract (2007) from the Spanish Science and Innovation Ministry. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Company of Biologists | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Melanocyte-stimulating hormone (MSH) | es_ES |
dc.subject | Proopiomelanocortin | es_ES |
dc.subject | MC5R | es_ES |
dc.subject | Phosphodiesterase inhibitor | es_ES |
dc.title | Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1242/jeb.035121 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1242/jeb.035121 | es_ES |
dc.identifier.e-issn | 1477-9145 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
Aparece en las colecciones: | (IATS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
characterization of the sea bass melanocortin.pdf | 612,19 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
40
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
38
checked on 28-feb-2024
Page view(s)
361
checked on 24-abr-2024
Download(s)
235
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.