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Open Access item Quantification of Manila clam ruditapes philippinarum (Adams & Reeve, 1850) larvae based on SYBR green real-time polymerase chain reaction

Authors:Quinteiro, Javier
Pérez-Diéguez, L.
Sánchez, Ana Cristina
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
Rey Méndez, Manuel
Keywords:Manila clam, Ruditapes philippinarum, 16S rRNA, Real-time PCR, SYBR Green, Larvae detection, Larvae quantification
Issue Date:2011
Publisher:National Shellfisheries Association
Citation:Journal of Shellfish Research 30(3): 791-796 (2011)
Abstract:Biological and ecological research is seriously handicapped because of difficulties experienced in the reliable detection and quantification of bivalve larvae. This is a critical issue in the case of the Manila clam Ruditapes philippinarum (Adams & Reeve, 1850), a largely invasive and commercially relevant species, with important wild, cultured, and naturalized populations throughout the world. A SYBR Green real-time PCR assay, containing TPHI16S1F and TPHI16S2R primers (specific to R. philippinarum female mtDNA), was designed and tested to provide a rapid and high-throughput PCR-based method for larval quantification. Accurate estimations of larval numbers in spiked plankton samples point to the usefulness of this system. It can be used through a wide range of evaluated variable experimental conditions, such as the presence of closely related bivalve species, stationary-dependent plankton abundance, sampling volumes, and larval size.
Description:6 páginas, 1 tabla, 5 figuras
Publisher version (URL):http://dx.doi.org/10.2983/035.030.0319
URI:http://hdl.handle.net/10261/47549
ISSN:0730-8000
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Appears in Collections:(IIM) Artículos

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