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Título: | Transcriptional regulators: Protein-DNA complexes and regulatory mechanisms |
Autor: | Rojo, Fernando CSIC ORCID ; Salas, Margarita CSIC ORCID | Palabras clave: | DNA Bacillus subtilis Phages Phi29 |
Fecha de publicación: | 1995 | Editor: | Elsevier | Citación: | Methods in Molecular Genetics 6: 421-438 | Resumen: | Processes such as DNA replication, transcription, and recombination involve the concerted action of diverse DNA-binding proteins acting on the DNA. Knowledge of the structure and working mechanisms of these nucleoprotein complexes has greatly improved since the 1980s, owing mainly to the appearance of a number of techniques that are relatively simple and give detailed information about the protein-DNA complexes. In this chapter we describe a number of these techniques that have been applied in the past years to study the switch from early to late transcription in Bacillus subtilis phage ~b29. We show the results obtained and describe how the techniques allowed us to elucidate the mechanism used by the transcriptional regulatory protein p4 to activate transcription from the viral late promoter. | Versión del editor: | http://dx.doi.org/10.1016/S1067-2389(06)80026-9 | URI: | http://hdl.handle.net/10261/47179 | DOI: | 10.1016/S1067-2389(06)80026-9 | ISSN: | 1064-3745 |
Aparece en las colecciones: | (CBM) Artículos |
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