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Título : Transcriptional regulators: Protein-DNA complexes and regulatory mechanisms
Autor : Rojo, Fernando, Salas, Margarita
Palabras clave : DNA
Bacillus subtilis
Fecha de publicación : 1995
Editor: Elsevier
Resumen: Processes such as DNA replication, transcription, and recombination involve the concerted action of diverse DNA-binding proteins acting on the DNA. Knowledge of the structure and working mechanisms of these nucleoprotein complexes has greatly improved since the 1980s, owing mainly to the appearance of a number of techniques that are relatively simple and give detailed information about the protein-DNA complexes. In this chapter we describe a number of these techniques that have been applied in the past years to study the switch from early to late transcription in Bacillus subtilis phage ~b29. We show the results obtained and describe how the techniques allowed us to elucidate the mechanism used by the transcriptional regulatory protein p4 to activate transcription from the viral late promoter.
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ISSN: 1064-3745
DOI: 10.1016/S1067-2389(06)80026-9
Citación : Methods in Molecular Genetics 6: 421-438
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