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Closed Access item Transcriptional regulators: Protein-DNA complexes and regulatory mechanisms

Authors:Rojo, Fernando
Salas, Margarita
Keywords:DNA, Bacillus subtilis, Phages, Phi29
Issue Date:1995
Citation:Methods in Molecular Genetics 6: 421-438
Abstract:Processes such as DNA replication, transcription, and recombination involve the concerted action of diverse DNA-binding proteins acting on the DNA. Knowledge of the structure and working mechanisms of these nucleoprotein complexes has greatly improved since the 1980s, owing mainly to the appearance of a number of techniques that are relatively simple and give detailed information about the protein-DNA complexes. In this chapter we describe a number of these techniques that have been applied in the past years to study the switch from early to late transcription in Bacillus subtilis phage ~b29. We show the results obtained and describe how the techniques allowed us to elucidate the mechanism used by the transcriptional regulatory protein p4 to activate transcription from the viral late promoter.
Publisher version (URL):http://dx.doi.org/10.1016/S1067-2389(06)80026-9
Appears in Collections:(CBM) Artículos

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